Active Recombinant Mouse Delta/Notch-Like EGF-Related Receptor

• Cat.No.: Dner-410M
• Product Overview: Recombinantmouse Dner protein was expressed in Murine myeloma cell line, NS0-derived. Ala26-His637.

Specification

• Cat. No.: Dner-410M
• Description: Dner is a type Itransmembrane glycoprotein of the Notch/Delta family. Mouse Dner has beendetected as 90, 120 and 150 kDa forms which are probably variablyglycosylated.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminal Sequence: Ala26
• Molecular Weight: 91.4 kDa
• Activity: Measured by itsability to inhibit Pleiotrophininduced neurite outgrowth of E16-E18 rat embryoniccortical neurons. When 4 x 104 neurons/well are added to 96well platecontaining serial dilutions of Recombinant Mouse DNER Fc Chimera andimmobilized Recombinant Mouse Pleiotrophin/PTN (1 μg/mL) neurite outgrowth issignificantly inhibited in a dose dependent manner. The ED50 forthis effect is typically 0.5-2.5 μg/mL.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 200μg/mL in PBS.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month,2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions afterreconstitution.

Gene Information

• Gene Name: Dner delta/notch-likeEGF-related receptor [ Mus musculus ]
• Official Symbol: Dner
• Synonyms: Dner; delta/notch-likeEGF-related receptor; BET; Bret; A930026D19Rik; MGC39095; delta andNotch-like epidermal growth factor-related receptor; transmembrane proteinBet; brain EGF repeat-containing transmembrane protein; Bet
• Gene ID: 227325
• mRNA Refseq: NM_152915
• Protein Refseq: NP_690879
• UniProt ID: Q8JZM4
• Chromosome Location: 1
• Function: receptor activity; calcium ion binding;Notch binding