||Recombinant human CCL3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer(24-92aa).
||CCL3, also known as, macrophage inflammatory protein-1alpha (MIP-1alpha), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This protein has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1beta and RANTES. It acts as an inhibitor of different strains of HIV-1, HIV-2 and SIV infection in a dose-dependent manner.
||Liquid. In 10mM Sodium Citrate ( pH3.5) containing 10% glycerol
||10.0 kDa (90aa), confirmed by MALDI-TOF(Molecular weight on SDS-PAGE will appear higher)
||> 90% by SDS - PAGE
||0.25 mg/ml (determined by Bradford assay)
||< 1.0 EU per 1 μg of protein (determined by LAL method )
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MSLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.