Active Recombinant Mouse Adhesion Molecule, Interacts With CXADR Antigen 1

• Cat.No.: Amica1-447M
• Product Overview: Recombinant Mouse Amica1protein was expressed in Murine myeloma cell line. Leu23-Leu281.

Specification

• Cat. No.: Amica1-447M
• Description: Amica1 is a 65kDa, type I transmembrane glycoprotein that belongs to the junctionaladhesion molecule (JAM) subset of the immunoglobulin superfamily. JAM familyproteins contribute to intercellular connections within epithelial andendothelial cell layers and mediate their interactions with varioushemopoietic cells. The mouse Amica1 cDNA encodes a 379amino acid (aa) precursor that includes a 20 aa signal sequence, a 261 aaextracellular domain (ECD) with two Iglike domains, a 21 aa transmembranesegment, and a 77 aa cytoplasmic domain. In contrast to other JAM familyproteins, AMICA does not contain a cytoplasmic PDZ binding motif.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 um filtered solution in PBS.
• N-terminalSequence: Leu23
• MolecularWeight: 56.6 kDa (monomer)
• Activity: Measuredby the ability of the immobilized protein to support the adhesion of human neutrophils.When 2 x 105 cells/well are added to rmAMICAcoated plates (10 μg/mL, 100μL/well), 30 50% of the cells will adhere after 20 minutes at 37°C.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• Purity: >95%,by SDSPAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 100μg/mLin sterile PBS.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, 20 to 70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, 20 to 70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Amica1 adhesion molecule,interacts with CXADR antigen 1 [ Mus musculus ]
• Official Symbol: Amica1
• Synonyms: Amica1; adhesionmolecule, interacts with CXADR antigen 1; AMICA; Crea7; Gm638;junctional adhesion molecule-like; mCrea7; adhesion molecule AMICA; dendriticcell-specific protein CREA7; dendritic-cell specific protein Crea7
• Gene ID: 270152
• mRNA Refseq: NM_001005421
• Protein Refseq: NP_001005421
• UniProt ID: Q80UL9
• Chromosome Location: 9
• Function: cell adhesionmolecule binding