"Cntn6" Related Products


Recombinant Mouse Contactin 6, His-tagged

Cat. No.: Cntn6-462M
Product Overview: Recombinant Mouse Cntn6protein was expressed in CHO using a C-terminal 6-His tag. Glu20-Ser998.
Description: Cntn6, also known asNB3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPIlinked neuronaladhesion proteins. Mature mouse Cntn6 consists of six immunoglobulinlike domains,a flexible linker region, four fibronectin type III domains, and a GPI anchor.Mouse Cntn6 shares 90% and 96% amino acid (aa) sequence identity with human andrat Cntn6, respectively. Alternate splicing generates an isoform with a 17 aadeletion in the first Iglike domain.
Source: Chinese Hamster Ovarycell line, CHO-derived
Form: Lyophilized from a 0.2μm filtered solution in PBS.
N-terminalSequence: Glu20
Molecular Weight: 109.2 kDa
Activity: Measuredby the ability of the immobilized protein to support the adhesion of Neuro2a mouseneuroblastoma cells (ATCC: CCL131). When 5 x 104 cells/well are addedto rmCntn6 coated plates (10 μg/mL, 100 μL/well), approximately 40-60% will adhereafter 30 minutes at 37 °C. Optimal dilutions should be determined by each laboratoryfor each application.
Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstituteat 100 μg/mL in sterile PBS
Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.
Gene Name: Cntn6 contactin6 [ Mus musculus ]
Official Symbol: Cntn6
Synonyms: Cntn6; contactin6; NB-3; mNB-3; neural recognition molecule NB-3
Gene ID: 53870
mRNA Refseq: NM_017383
Protein Refseq: NP_059079
UniProt ID: Q9JMB8
Chromosome Location: 6; 6 E2

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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