"Col13a1" Related Products

Recombinant Mouse Collagen, Type XIII, Alpha 1, His-tagged

Cat. No.: Col13a1-465M
Product Overview: Recombinant Mouse Col13a1protein was expressed in Murine myeloma cell line with a N-terminal 6-His tag.Glu107-Gln565.
Description: Col13a1 is an 85 kDa95 kDa protein in the type 2 transmembrane collagen family. Mature mouse Col13a1consists of a 40 amino acid (aa) cytoplasmic domain, a 19 aa transmembrane segment,and a 692 aa extracellular domain (ECD). The ECD contains three collagenous regionsseparated by shorter noncollagenous regions. Within comparable regions of theECD, mouse Col13a1 shares 85% and 88% aa sequence identity with human and ratCol13a1, respectively.
Source: Murine myeloma cell line,NS0-derived
Form: Lyophilized from a 0.2μm filtered solution in PBS.
N-terminalSequence: His
Molecular Weight: 46.0 kDa
Activity: Measuredby the ability of the immobilized protein to support the adhesion of A431 humanepidermoid carcinoma cells. When 1 x 105 cells per well are added tormCol13a1 coated plate (2 μg/mL, 100 μL/well), approximately 45%-75% will adhereafter 1 hour at 37°C. Optimal dilutions should be determined by each laboratoryfor each application.
Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
Purity: >80%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstituteat 300 μg/mL in PBS
Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.
Gene Name: Col13a1 collagen,type XIII, alpha 1 [ Mus musculus ]
Official Symbol: Col13a1
Synonyms: Col13a1; collagen,type XIII, alpha 1; collagen alpha-1(XIII) chain; type XIII collagen; procollagen,type XIII, alpha 1
Gene ID: 12817
mRNA Refseq: NM_007731
Protein Refseq: NP_031757
UniProt ID: Q9R1N9
Chromosome Location: 10 B4; 10 31.5 cM
Function: heparin binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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