"EPYC" Related Products


Recombinant Mouse Epiphycan, His-tagged

Cat. No.: Epyc-467M
Product Overview: Recombinant Mouse Epycprotein was expressed in Murine myeloma cell line with a C-terminal 10-His tag.Ala20-Ile322.
Description: Epyc, also called epiphycanor PGLb, is a 95-100 kDa class III subfamily member of the SLRP (small leucinerichproteoglycans) family of molecules. It is synthesized as a 322 amino acid (aa)precursor that contains a 19 aa signal sequence and a 303 aa mature region. Themature molecule contains three N-terminal O-glycosylation sites (including onefound to bind dermatin sulfate) and a glutamic acidrich motif followed by sixleucinerich repeats (LRR) that contain two potential Nglycosylation sites.
Source: Murine myeloma cell line,NS0-derived
Form: Lyophilized from a 0.2μm filtered solution in PBS.
N-terminalSequence: Ala20
Molecular Weight: 36.2 kDa
Activity: Measuredby the ability of the immobilized protein to inhibit the adhesion of SAOS2 humanosteosarcoma cells (ATCC: HTB85) to human Fibronectin. When 5 x 104cells/well are added to Mouse Epyc and Human Fibronectin(0.5 μg/mL) coated plates, the cell adhesion is enhanced in a dose dependent manner.The ED50 for this effect is typically 0.3-1.2 μg/mL. Optimal dilutionsshould be determined by each laboratory for each application.
Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstituteat 200 μg/mL in PBS
Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.
Gene Name: Epyc epiphycan[ Mus musculus ]
Official Symbol: Epyc
Synonyms: Epyc; epiphycan;Dspg3; PG-Lb; SLRR3B; proteoglycan-Lb; dermatan sulfate proteoglycan 3; dermatansulphate proteoglycan 3; small chondroitin/dermatan sulfate proteoglycan; Pglb
Gene ID: 13516
mRNA Refseq: NM_007884
Protein Refseq: NP_031910
UniProt ID: P70186
Chromosome Location: 10 C2-C3; 10 55.0 cM

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.