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Recombinant Mouse Epiphycan, His-tagged

Cat. No.: Epyc-467M
Product Overview: Recombinant Mouse Epycprotein was expressed in Murine myeloma cell line with a C-terminal 10-His tag.Ala20-Ile322.
Description: Epyc, also called epiphycanor PGLb, is a 95-100 kDa class III subfamily member of the SLRP (small leucinerichproteoglycans) family of molecules. It is synthesized as a 322 amino acid (aa)precursor that contains a 19 aa signal sequence and a 303 aa mature region. Themature molecule contains three N-terminal O-glycosylation sites (including onefound to bind dermatin sulfate) and a glutamic acidrich motif followed by sixleucinerich repeats (LRR) that contain two potential Nglycosylation sites.
Source: Murine myeloma cell line,NS0-derived
Form: Lyophilized from a 0.2μm filtered solution in PBS.
N-terminalSequence: Ala20
Molecular Weight: 36.2 kDa
Activity: Measuredby the ability of the immobilized protein to inhibit the adhesion of SAOS2 humanosteosarcoma cells (ATCC: HTB85) to human Fibronectin. When 5 x 104cells/well are added to Mouse Epyc and Human Fibronectin(0.5 μg/mL) coated plates, the cell adhesion is enhanced in a dose dependent manner.The ED50 for this effect is typically 0.3-1.2 μg/mL. Optimal dilutionsshould be determined by each laboratory for each application.
Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstituteat 200 μg/mL in PBS
Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.
Gene Name: Epyc epiphycan[ Mus musculus ]
Official Symbol: Epyc
Synonyms: Epyc; epiphycan;Dspg3; PG-Lb; SLRR3B; proteoglycan-Lb; dermatan sulfate proteoglycan 3; dermatansulphate proteoglycan 3; small chondroitin/dermatan sulfate proteoglycan; Pglb
Gene ID: 13516
mRNA Refseq: NM_007884
Protein Refseq: NP_031910
UniProt ID: P70186
Chromosome Location: 10 C2-C3; 10 55.0 cM

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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