| Species : |
Mouse |
| Source : |
Mammalian Cells |
| Tag : |
His |
| Protein Length : |
20-322 a.a. |
| Description : |
Epyc, also called epiphycanor PGLb, is a 95-100 kDa class III subfamily member of the SLRP (small leucinerichproteoglycans) family of molecules. It is synthesized as a 322 amino acid (aa)precursor that contains a 19 aa signal sequence and a 303 aa mature region. Themature molecule contains three N-terminal O-glycosylation sites (including onefound to bind dermatin sulfate) and a glutamic acidrich motif followed by sixleucinerich repeats (LRR) that contain two potential Nglycosylation sites. |
| Form : |
Lyophilized from a 0.2μm filtered solution in PBS. |
| N-terminalSequence : |
Ala20 |
| Molecular Weight : |
36.2 kDa |
| Activity : |
Measuredby the ability of the immobilized protein to inhibit the adhesion of SAOS2 humanosteosarcoma cells (ATCC: HTB85) to human Fibronectin. When 5 x 104cells/well are added to Mouse Epyc and Human Fibronectin(0.5 μg/mL) coated plates, the cell adhesion is enhanced in a dose dependent manner.The ED50 for this effect is typically 0.3-1.2 μg/mL. Optimal dilutionsshould be determined by each laboratory for each application. |
| Endotoxin Level : |
<1.0 EUper 1 μg of the protein by the LAL method. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstituteat 200 μg/mL in PBS |
| Storage : |
Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution. |
