||Recombinant humanERCC1 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography.
||ERCC1, also known asDNA excision repair protein ERCC-1, functions in the nucleotide excisionrepair pathway, and is required for the repair of DNA lesions such as thoseinduced by UV light or formed by electrophilic compounds including cisplatin.Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type4 (COFS4).
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
||32.5 kDa (297aa),confirmed by MALDI-TOF
||> 90% by SDS -PAGE
||0.5 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMDPGKD KEGVPQPSGP PARKKFVIPL DEDEVPPGVA KPLFRSTQSL PTVDTSAQAAPQTYAEYAIS QPLEGAGATC PTGSEPLAGE TPNQALKPGA KSNSIIVSPR QRGNPVLKFV RNVPWEFGDVIPDYVLGQST CALFLSLRYH NLHPDYIHGR LQSLGKNFAL RVLLVQVDVK DPQQALKELA KMCILADCTLILAWSPEEAG RYLETYKAYE QKPADLLMEK LEQDFVSRSL EQLIAASRED LALCPGLGPQ KARRLFDVLHEPFLKVP
||Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.