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Recombinant E. coli HchA, His-tagged

Cat.No. : hchA-3513E
Product Overview : Recombinant E. colihchA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-283aa).
  • Specification
  • Gene Information
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Cat. No. : hchA-3513E
Description : HchA, also known as,Escherichia coli Hsp31, is a homodimeric member of the ThiI/DJ-1/PfpI superfamily that combines molecular chaperone and aminopeptidase activities. It uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress.
Form : Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol0.1M NaCl,1mM DTT
Molecular Weight : 33.3 kDa(303aa) confirmed by MALDI-TOF
Purity : > 95% by SDS - PAGE
Concentration : 1.0 mg/ml (determined by Bradford assay)
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MTVQTSKNPQ VDIAEDNAFF PSEYSLSQYT SPVSDLDGVD YPKPYRGKHK ILVIAADERY LPTDNGKLFSTGNHPIETLL PLYHLHAAGF EFEVATISGL MTKFEYWAMP HKDEKVMPFF EQHKSLFRNP KKLADVVASL NADSEYAAIF VPGGHGALIGLPESQDVAAA LQWAIKNDRF VISLCHGPAA FLALRHGDNP LNGYSICAFP DAADKQTPEI GYMPGHLTWY FGEELKKMGM NIINDDITGRVHKDRKLLTG DSPFAANALG KLAAQEMLAA YAG
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
Gene Name : hchA Hsp31 molecular chaperone [ Escherichia coli str. K-12 substr. MG1655 ]
Synonyms : Hsp31 molecular chaperone; ECK1963; JW1950; yedU; yzzC
Gene ID : 946481
Protein Refseq : NP_416476

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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