| Species : |
E.coli |
| Source : |
E.coli |
| Tag : |
His |
| Description : |
HchA, also known as,Escherichia coli Hsp31, is a homodimeric member of the ThiI/DJ-1/PfpI superfamily that combines molecular chaperone and aminopeptidase activities. It uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. |
| Form : |
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol0.1M NaCl,1mM DTT |
| Molecular Weight : |
33.3 kDa(303aa) confirmed by MALDI-TOF |
| Purity : |
> 95% by SDS - PAGE |
| Concentration : |
1.0 mg/ml (determined by Bradford assay) |
| Sequences of amino acids : |
MGSSHHHHHH SSGLVPRGSH MTVQTSKNPQ VDIAEDNAFF PSEYSLSQYT SPVSDLDGVD YPKPYRGKHK ILVIAADERY LPTDNGKLFSTGNHPIETLL PLYHLHAAGF EFEVATISGL MTKFEYWAMP HKDEKVMPFF EQHKSLFRNP KKLADVVASL NADSEYAAIF VPGGHGALIGLPESQDVAAA LQWAIKNDRF VISLCHGPAA FLALRHGDNP LNGYSICAFP DAADKQTPEI GYMPGHLTWY FGEELKKMGM NIINDDITGRVHKDRKLLTG DSPFAANALG KLAAQEMLAA YAG |
| Storage : |
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles. |
