||Recombinant mouse Ighgprotein, was expressed in Hi-5 cell using baculovirus expression system andpurified by using conventional chromatography techniques.
||ImmunoglobulinG (IgG) are antibody molecules. Each Ighg iscomposed of four peptide chains (two heavy chains γ and two light chains).Each Ighg has two antigen binding sites. Ighg antibodies are involved in predominantly the secondaryimmune response. The presence of specific Ighg,in general, corresponds to maturation of the antibody response. It plays animportant role in Antibody-dependent cell-mediated cytotoxicity (ADCC) andIntracellular antibody-mediated proteolysis, in which it binds to TRIM21 (thereceptor with greatest affinity to Ighg inhumans) in order to direct marked virions to the proteasome in the cytosol.
||Liquid. In 20mMTris-HCl buffer (pH 8.0).
||> 90%by SDS-PAGE
||0.5mg/ml (determined by Bradford assay)
|Sequences of aminoacids:
||ADPEPRGPTIKPCPPCKCPA PNLLGGPSVF IFPPKIKDVL MISLSPIVTC VVVDVSEDDP DVQISWFVNN VEVHTAQTQTHREDYNSTLRVVSALPIQHQ DWMSGKEFKC KVNNKDLPAP IERTISKPKG SVRAPQVYVL PPPEEEMTKKQVTLTCMVTD FMPEDIYVEW TNNGKTELNYKNTEPVLDSD GSYFMYSKLR VEKKNWVERN SYSCSVVHEGLHNHHTTKSF SRTPGK
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.