Penicillin G amidase

• Cat.No.: CEZ054

Specification

• Species: Bacillus megaterium
• Source: Bacillus Megaterium
• Tag: Non
• Description: Penicillin G amidase is an enzyme that catalyzes the chemical reaction:penicillin + H2O a carboxylate + 6-aminopenicillanate Thus, the two substrates of this enzyme are penicillin and H2O, whereas its two products are carboxylate and 6-aminopenicillanate. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than petide bonds, specifically in linear amides. This enzyme participates in penicillin and cephalosporin biosynthesis.
• Enzyme Commission Number: EC 3.5.1.11
• Synonym: Penicillin amidohydrolase; penicillin acylase; benzylpenicillin acylase; novozym 217; semacylase; alpha-acylamwino-beta-lactam acylhydrolase; ampicillin acylase.
• Physical form: Spherically Formed White Pellets.
• Carrier: Polymethacrylate Polymer.
• Carrier Immobilisation: Covalent Cross Linking.
• Particle Size: 100-300μm.
• Dry Weight: around 44%.
• pH Stability: 6.0~8.5.
• Optimum pH: 8.0.
• Optimum temperature: 55°C
• Specific activity: ≥ 240U/g.
• Substrate Concentration: 8.0%.
• Stability: Minimum 18 months subject to specified conditions.
• Storage: Below 4°C and isolated from air.