Recombinant Human ANXA5 protein ANXA5-6861H

Recombinant Human ANXA5 protein

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Recombinant Human ANXA5 protein

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Cat.No. : ANXA5-6861H
Product Overview : Recombinant Human ANXA5 (Met1-Asp320) protein carried no tag and was expressed in E.coli.
Description : Anexin A5 is a phospholipid binding protein, which binds with high affinity and selectivity to PS in the presence of calcium. PS is predominantly located in membrane leaflets, which face the cytosol. However, recent findings show that each cell type has the molecular machinery to expose PS at its cell surface. This machinery is activated during the execution of apoptosis. Once PS is exposed at the cell surface it exhibits procoagulant and proinflammatory activities. Anexin A5 will bind to the PS-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. Anexin A5 has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Anexin A5 is associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).
Source : E.coli
Species : Human
Predicted N Terminal : Met1
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 36 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Met1-Asp320
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >98% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of ANXA5-6861H :

Human ANXA5, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Gene Name : ANXA5
Official Symbol : ANXA5
Synonyms : ANXA5; annexin A5; ANX5, ENX2; CBP-I; PAP-I; VAC-alpha; annexin V; annexin-5; anchorin CII; endonexin II; lipocortin V; calphobindin I; thromboplastin inhibitor; vascular anticoagulant-alpha; placental anticoagulant protein 4; placental anticoagulant protein I; PP4; ANX5; ENX2
Gene ID : 308
mRNA Refseq : NM_001154
Protein Refseq : NP_001145
MIM : 131230
UniProt ID : P08758

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