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Mouse Anti-Human CD80 Molecule Monoclonal Antibody

Cat.No. : CAB11503MH
Product Overview : Mouse monoclonal antibody to human CD80 molecule.
  • Specification
  • Gene Information
  • Related Products
Antigen Description : The B-lymphocyte activation antigen B7-1(referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. Binding of this protein with CTLA-4 is involved in down-regulation of immune response.Recent study has revealed that contacts between B and T helper cells mediated by CD80 provide negative signal for the proliferation and IgG secretion of normal B cells and B cell lymphomas. In addition, B7-1 also plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages.CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
Specificity : Human CD80 / B7-1. No cross-reactivity in ELISA with Human CD28, Human B7-2, Human B7-H1, Mouse B7-H2, Human PDL2, Human cell lysate (293 cell line).
Immunogen : Recombinant Human CD80 / B7-1 Protein
Host : Mouse
Isotype : Mouse IgG1
Species : Human
Clone : 3G2D5
Purification : Protein A affinity chromatography
Applications : Western Blot; ELISA
Dilution : Western blot : This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human B7-1 in WB. Using a DAB detection system, the detection limit for Human B7-1 is approximately 20 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.5 - 1 μg with the appropriate secondary reagents to detect Human B7-1. The detection limit for Human B7-1 is approximately 0.00975 ng/well.
Preparation : This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human CD80 / B7-1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format : 0.2 μm filtered solution in PBS with 5% trehalose
Storage : This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name : CD80 CD80 molecule [ Homo sapiens ]
Official Symbol : CD80
Synonyms : CD80; CD80 molecule; CD28LG, CD28LG1,CD80 antigen (CD28 antigen ligand 1, B7 1 antigen) ,CD80 molecule; T-lymphocyte activation antigen CD80; B lymphocyte activation antigen B7; B7 1; B7.1; activation B7-1 antigen; costimulatory factor CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; costimulatory molecule variant IgV-CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); B7; BB1; B7-1; LAB7; CD28LG; CD28LG1;
Gene ID : 941
mRNA Refseq : NM_005191
Protein Refseq : NP_005182
MIM : 112203
UniProt ID : P33681
Chromosome Location : 3q13.3-q21
Pathway : Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem;
Function : coreceptor activity; protein binding; receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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