Mouse Anti-Human Cadherin 12, Type 2 Monoclonal Antibody


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Mouse Anti-Human Cadherin 12, Type 2 Monoclonal Antibody

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Cat.No. : CAB11506MH
Product Overview : Mouse Monoclonal Antibody to Human Cadherin 12, Type 2
Antigen Description : Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. They play important roles in cell adhesion, ensuring that cells within tissues are bound together. They are dependent on calcium(Ca2+) ions to function. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development. Cadherin-12 also known as CDH12, is a type I I classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherin-12 appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis.
Specificity : Human Cadherin-12 / CDH12. No cross-reactivity in ELISA with Human Cadherin-4; Human Cadherin-6; Human Cadherin-8; Human E-Cadherin; Human cell lysate (293 cell line)
Immunogen : Recombinant Human Cadherin-12 / CDH12 Protein
Host : Mouse
Isotype : N/A
Species : Human
Clone : 5I9H5
Applications : WB; ELISA
Dilution : Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CDH12 in WB. Using a DAB detection system, the detection limit for Human CDH12 is approximately 20 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CDH12. The detection limit for Human CDH12 is approximately 0.078 ng/well.
Preparation : This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human Cadherin-12 / CDH12. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format : 0.2 μm filtered solution in PBS with 5% trehalose
Storage : This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name : CDH12 cadherin 12, type 2 (N-cadherin 2) [ Homo sapiens ]
Official Symbol : CDH12
Synonyms : CDH12; cadherin 12, type 2 (N-cadherin 2); cadherin-12; Br cadherin; CDHB; Br-cadherin; N-cadherin 2; brain cadherin; brain-cadherin; neuronal cadherin 2; neural type cadherin 2; cadherin, neural type, 2; FLJ34857;
Gene ID : 1010
mRNA Refseq : NM_004061
Protein Refseq : NP_004052
MIM : 600562
UniProt ID : P55289
Chromosome Location : 5p14.3
Pathway : Adherens junctions interactions, organism-specific biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem;
Function : calcium ion binding;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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