||Recombinant human SNRNP25 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-132aa).
||SNRNP25 belongs to the small nuclear ribonucleoprotein core protein family. This protein is a component of the U12-type spliceosome that is found in some eukaryotesand removes U12-type introns, which are rare and have distinctsplice consensus signals. The U12-type spliceosome consists ofseveral small nuclear RNAs and associated proteins.
||Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol, 2mM DTT, 0.2M NaCl.
||17.4 kDa (152aa) confirmed by MALDI-TOF
||>85 % by SDS -PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MDVFQEGLAM VVQDPLLCDL PIQVTLEEVN SQIALEYGQA MTVRVCKMDG EVMPVVVVQS ATVLDLKKAI QRYVQLKQER EGGIQHISWS YVWRTYHLTS AGEKLTEDRK KLRDYGIRNR DEVSFIKKLR QK
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.