Active Recombinant Human IFNA7 protein(Cys24-Asp189), hFc-tagged

• Cat.No.: IFNA7-176H
• Product Overview: Recombinant Human IFNA7 (P01567) (Cys 24-Asp 189) was expressed in HEK293, fused with the Fc region of Human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: N-hFc
• Protein length: Cys24-Asp189
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 0.1-1 ng/mL.
• Molecular Mass: The recombinant human IFNA7/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 427 amino acids with a predicted molecular mass of 48 kDa. rh IFNA7/Fc monomer migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IFNA7 interferon, alpha 7 [ Homo sapiens ]
• Official Symbol: IFNA7
• Synonyms: IFNA7; interferon, alpha 7; interferon alpha-7; IFN alphaJ; IFNA J; leIF J; IFN-alpha 7; IFN-alpha-7; IFN-alpha-J1; interferon alpha-J; interferon alpha-J1; IFNA-J; IFN-alphaJ
• Gene ID: 3444
• mRNA Refseq: NM_021057
• Protein Refseq: NP_066401
• MIM: 147567
• UniProt ID: P01567