Recombinant Human Signaling Threshold Regulating Transmembrane Adaptor 1, His-tagged

• Cat.No.: SIT1-6861H
• Product Overview: Recombinant human SIT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 16.9 kDa.

Specification

• Description: SIT1, also known as signaling threshold-regulating transmembrane adapter 1, negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. This protein involved in positive selection of T-cells.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol.
• Molecular Mass: 16.9 kDa (156aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher).
• Protein length: 1-196aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MHLSQWTRGR SRSHPGQGRS GESVEEVPLY GNLHYLQTGR LSQDPEPDQQ DPTLGGPARA AEEVMCYTSL QLRPPQGRIP GPGTPVKYSE VVLDSEPKSQ ASGPEPELYA SVCAQTRRAR ASFPDQAYAN SQPAAS
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SIT1 signaling threshold regulating transmembrane adaptor 1 [ Homo sapiens ]
• Official Symbol: SIT1
• Synonyms: SIT1; signaling threshold regulating transmembrane adaptor 1; suppression inducing transmembrane adaptor 1; signaling threshold-regulating transmembrane adapter 1; SHP2 interacting transmembrane adaptor; SIT; gp30/40; suppression-inducing transmembrane adapter 1; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; RP11-331F9.5; MGC125908; MGC125909; MGC125910
• Gene ID: 27240
• mRNA Refseq: NM_014450
• Protein Refseq: NP_055265
• MIM: 604964
• UniProt ID: Q9Y3P8
• Chromosome Location: 9p13-p12
• Pathway: T Cell Receptor Signaling Pathway, organism-specific biosystem
• Function: SH2 domain binding; kinase binding; protein binding