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Recombinant Human Nth Endonuclease III-Like 1 (E. coli), His-tagged

Cat.No. : NTHL1-2488H
Product Overview : Recombinant human NTHL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 36.6 kDa.
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Description : NTHL1 is one of the enzyme that has been shown to act on a large number of oxidatively damaged DNA sites. It has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. It acts on a damaged strand, 5" from the damaged site and incises damaged DNA at cytosines, thymines and guanines.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT.
Molecular Mass : 36.6 kDa (332aa), confirmed by MALDI-TOF.
Protein length : 1-312aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MCSPQESGMT ALSARMLTRS RSLGPGAGPR GCREEPGPLR RREAAAEARK SHSPVKRPRK AQRLRVAYEG SDSEKGEGAE PLKVPVWEPQ DWQQQLVNIR AMRNKKDAPV DHLGTEHCYD SSAPPKVRRY QVLLSLMLSS QTKDQVTAGA MQRLRARGLT VDSILQTDDA TLGKLIYPVG FWRSKVKYIK QTSAILQQHY GGDIPASVAE LVALPGVGPK MAHLAMAVAW GTVSGIAVDT HVHRIANRLR WTKKATKSPE ETRAALEEWL PRELWHEING LLVGFGQQTC LPVHPRCHAC LNQALCPAAQ GL
Purity : >80% by SDS - PAGE
Applications : SDS-PAGE
Storage : Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration : 0.25 mg/ml (determined by Bradford assay)
Gene Name : NTHL1 nth endonuclease III-like 1 (E. coli) [ Homo sapiens ]
Official Symbol : NTHL1
Synonyms : NTHL1; nth endonuclease III-like 1 (E. coli); nth (E.coli endonuclease III) like 1; endonuclease III-like protein 1; NTH1; OCTS3;
Gene ID : 4913
mRNA Refseq : NM_002528
Protein Refseq : NP_002519
MIM : 602656
UniProt ID : P78549
Chromosome Location : 16p13.3
Pathway : Base Excision Repair, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Base-Excision Repair, AP Site Formation, organism-specific biosystem; Base-free sugar-phosphate removal via the single-nucleotide replacement pathway, organism-specific biosystem; Cleavage of the damaged pyrimidine, organism-specific biosystem; DNA Repair, organism-specific biosystem;
Function : 4 iron, 4 sulfur cluster binding; DNA N-glycosylase activity; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; double-stranded DNA binding; endonuclease activity; hydrolase activity, acting on glycosyl bonds; lyase activity; metal ion binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Are there any genetic counseling considerations for individuals with NTHL1 mutations? 08/27/2019

Individuals with NTHL1 mutations may benefit from genetic counseling to understand their cancer risks and make informed decisions about their healthcare.

Can NTHL1 mutations be used as a predictive biomarker for cancer risk? 06/15/2018

Yes, individuals with NTHL1 mutations may have an increased risk of developing certain types of cancer, making it a potential biomarker for cancer risk assessment.

How does NTHL1 protein contribute to the DNA repair process? 01/28/2018

NTHL1 protein is involved in the base excision repair pathway, where it removes damaged DNA bases and initiates the repair process.

How are NTHL1 mutations inherited and what is the likelihood of passing them on to future generations? 01/28/2017

NTHL1 mutations can be inherited in an autosomal recessive manner, meaning that both parents need to carry a mutated copy of the gene to pass it on to their children.

Are there any specific populations or ethnic groups that are more susceptible to NTHL1 mutations? 09/17/2016

Studies have suggested that individuals of Ashkenazi Jewish descent may have a higher prevalence of NTHL1 mutations and associated cancer risks.

Customer Reviews (3)

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Reviews
07/08/2022

    With its well-characterized nature and availability in various formats, the arnC protein is an advantageous tool that aids researchers in achieving their scientific goals.

    06/14/2022

      The manufacturer also ensures that the NTHL1 protein is well-characterized, with extensive documentation on its functional properties.

      11/18/2020

        Whether it's troubleshooting issues, optimizing experimental protocols, or providing detailed information about the protein, the manufacturer's support team is dedicated to helping researchers overcome any challenges they may encounter.

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