Recombinant Human SIL1 Homolog, Endoplasmic Reticulum Chaperone (S. cerevisiae), His-tagged

• Cat.No.: SIL1-6832H
• Product Overview: Recombinant human SIL1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: SIL1 is a resident endoplasmic reticulum, N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol.
• Molecular Mass: 50 kDa (439aa)
• Protein length: 32-461aa
• AA Sequence: MHQNLKEFAL TNPEKSSTKE TERKETKAEE ELDAEVLEVF HPTHEWQALQ PGQAVPAGSH VRLNLQTGER EAKLQYEDKF RNNLKGKRLD INTNTYTSQD LKSALAKFKE GAEMESSKED KARQAEVKRL FRPIEELKKD FDELNVVIET DMQIMVRLIN KFNSSSSSLE EKIAALFDLE YYVHQMDNAQ DLLSFGGLQV VINGLNSTEP LVKEYAAFVL GAAFSSNPKV QVEAIEGGAL QKLLVILATE QPLTAKKKVL FALCSLLRHF PYAQRQFLKL GGLQVLRTLV QEKGTEVLAV RVVTLLYDLV TEKMFAEEEA ELTQEMSPEK LQQYRQVHLL PGLWEQGWCE ITAHLLALPE HDAREKVLQT LGVLLTTCRD RYRQDPQLGR TLASLQAEYQ VLASLELQDG EDEGYFQELL GSVNSLLKEL RLEHHHHHH
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SIL1 SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: SIL1
• Synonyms: SIL1; SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae); Marinesco Sjogren syndrome , MSS; nucleotide exchange factor SIL1; BAP; ULG5; BiP-associated protein; MSS
• Gene ID: 64374
• mRNA Refseq: NM_001037633
• Protein Refseq: NP_001032722
• MIM: 608005
• UniProt ID: Q9H173
• Chromosome Location: 5q31
• Pathway: Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem
• Function: binding; unfolded protein binding