"ATPIF1" Related Products

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Recombinant Human ATPase Inhibitory Factor 1, His-tagged

Cat.No.: ATPIF1-2517H
Product Overview: Recombinant human ATPIF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Description: ATPIF1 binds to the C-terminal region of a beta subunit of the F1-ATPase at low pH values and, via interference of the beta and gamma subunit interaction, ATPIF1 regulates the activity of the F1, F0 ATPase. The overexpression of ATPIF1 in several human carcinomas further supports its participation in oncogenesis and provides insight into the altered metabolism of cancer cells, which includes the reprogramming of energetic metabolism toward glycolysis.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT.
Molecular Mass: 12.2 kDa (106aa), confirmed by MALDI-TOF
Protein length: 26-106aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGSDQS ENVDRGAGSI REAGGAFGKR EQAEEERYFR AQSREQLAAL KKHHEEEIVH HKKEIERLQK EIERHKQKIK MLKHDD
Purity: >95% by SDS - PAGE
Applications: SDS-PAGE
Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration: 1 mg/ml (determined by Bradford assay)
Gene Name: ATPIF1 ATPase inhibitory factor 1 [ Homo sapiens ]
Official Symbol: ATPIF1
Synonyms: ATPIF1; ATPase inhibitory factor 1; ATPase inhibitor, mitochondrial; ATP synthase inhibitor protein; ATPase inhibitor protein; ATPI; ATPIP; IP; MGC1167; MGC8898; IF1; IF(1); inhibitor of F(1)F(o)-ATPase;
Gene ID: 93974
mRNA Refseq: NM_016311
Protein Refseq: NP_057395
UniProt ID: Q9UII2
Chromosome Location: 1p35.3
Pathway: Electron Transport Chain, organism-specific biosystem;
Function: ATPase binding; ATPase binding; ATPase inhibitor activity; ATPase inhibitor activity; angiostatin binding; calmodulin binding; enzyme inhibitor activity; protein homodimerization activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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