Recombinant Human ADP-Ribosylarginine Hydrolase, His-tagged

• Cat.No.: ADPRH-2518H
• Product Overview: Recombinant human ADPRH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: ADPRH belongs to the ADP-ribosylglycohydrolase family. This protein catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. Unlike the rat and mouse enzymes, which require DTT for maximal activity, the human enzyme is DTT-independent.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 100mM NaCl.
• Molecular Mass: 42.1 kDa (381aa), confirmed by MALDI-TOF
• Protein length: 1-357aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMEKYVA AMVLSAAGDA LGYYNGKWEF LQDGEKIHRQ LAQLGGLDAL DVGRWRVSDD TVMHLATAEA LVEAGKAPKL TQLYYLLAKH YQDCMEDMDG RAPGGASVHN AMQLKPGKPN GWRIPFNSHE GGCGAAMRAM CIGLRFPHHS QLDTLIQVSI ESGRMTHHHP TGYLGALASA LFTAYAVNSR PPLQWGKGLM ELLPEAKKYI VQSGYFVEEN LQHWSYFQTK WENYLKLRGI LDGESAPTFP ESFGVKERDQ FYTSLSYSGW GGSSGHDAPM IAYDAVLAAG DSWKELAHRA FFHGGDSDST AAIAGCWWGV MYGFKGVSPS NYEKLEYRNR LEETARALYS LGSKEDTVIS L
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ADPRH ADP-ribosylarginine hydrolase [ Homo sapiens ]
• Official Symbol: ADPRH
• Synonyms: ADPRH; ADP-ribosylarginine hydrolase; [Protein ADP-ribosylarginine] hydrolase; ARH1; ADP-ribose-L-arginine cleaving enzyme
• Gene ID: 141
• mRNA Refseq: NM_001125
• Protein Refseq: NP_001116
• MIM: 603081
• UniProt ID: P54922
• Chromosome Location: 3q13.31-q13.33
• Function: ADP-ribosylarginine hydrolase activity; hydrolase activity; magnesium ion binding