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Recombinant Human Tetratricopeptide Repeat Domain 1, His-tagged

Cat.No.: TTC1-6953H
Product Overview: Recombinant human TTC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Description: TTC1 is composed of 292 amino acids with three TPR motifs, as a Galpha16-binding protein. The interaction was confirmed both in vitro and in transfected mammalian cells, where TTC1 also binds to several other Galpha proteins. TTC1 was found to interact with Ha-Ras preferentially in its active form.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT.
Molecular Mass: 36.1 kDa (316aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher).
Protein length: 1-292aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGEKSE NCGVPEDLLN GLKVTDTQEA ECAGPPVPDP KNQHSQSKLL RDDEAHLQED QGEEECFHDC SASFEEEPGA DKVENKSNED VNSSELDEEY LIELEKNMSD EEKQKRREES TRLKEEGNEQ FKKGDYIEAE SSYSRALEMC PSCFQKERSI LFSNRAAARM KQDKKEMAIN DCSKAIQLNP SYIRAILRRA ELYEKTDKLD EALEDYKSIL EKDPSIHQAR EACMRLPKQI EERNERLKEE MLGKLKDLGN LVLRPFGLST ENFQIKQDSS TGSYSINFVQ NPNNNR
Purity: >85% by SDS - PAGE
Applications: SDS-PAGE
Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration: 1 mg/ml (determined by Bradford assay)
Gene Name: TTC1 tetratricopeptide repeat domain 1 [ Homo sapiens ]
Official Symbol: TTC1
Synonyms: TTC1; tetratricopeptide repeat domain 1; tetratricopeptide repeat protein 1; TPR1; TPR repeat protein 1; FLJ46404;
Gene ID: 7265
mRNA Refseq: NM_003314
Protein Refseq: NP_003305
MIM: 601963
UniProt ID: Q99614
Chromosome Location: 5q32-q33.2
Function: protein binding; unfolded protein binding;

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