Active Recombinant Human LATS1, GST-tagged

• Cat.No.: LATS1-1423H
• Product Overview: Recombinant human LATS1 (589-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 589 aa-end
• Description: LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis which is phosphorylated in a cell-cycle dependent manner. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity which indicating a role as a negative regulator of CDC2/cyclin A and the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. LATS1 act as a tumor suppressor and play an important role in the development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 1.4 nmol/min/mg
• Molecular Mass: ~95 kDa
• Purity: >75%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: LATS1 LATS, large tumor suppressor, homolog 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: LATS1
• Synonyms: LATS1; LATS, large tumor suppressor, homolog 1 (Drosophila); LATS (large tumor suppressor, Drosophila) homolog 1; serine/threonine-protein kinase LATS1; WARTS; h-warts; WARTS protein kinase; large tumor suppressor homolog 1; wts
• Gene ID: 9113
• mRNA Refseq: NM_004690
• Protein Refseq: NP_004681
• MIM: 603473
• UniProt ID: O95835
• Chromosome Location: 6q25.1
• Pathway: Signal Transduction, organism-specific biosystem; Signaling by Hippo, organism-specific biosystem
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein binding; protein kinase binding; protein serine/threonine kinase activity