Active Recombinant Human PI4K2A, GST-tagged

• Cat.No.: PI4K2A-1450H
• Product Overview: Recombinant full-length human PI4K2A was co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PI4K2A or phosphatidylinositol 4-kinase type 2 alpha is involved in the formation of phosphatidylinositolpolyphosphates (PtdInsPs) which are centrally involved in many biologic processes ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4K2A phosphorylates PtdIns at the D-4 position which is an essential step in the biosynthesis of PtdInsPs. PI4K2A is a novel regulator of tumor growth by its action on angiogenesis and HIF-1alpha regulation. PI4K2A generates PtdIns4P-rich domains within the Golgi which regulate targeting of clathrin adaptor AP-1 complexes. PI4K2A is also required for Wnt to regulate LRP6 phosphorylation.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 95 nmol/min/mg
• Molecular Mass: ~79 kDa
• Protein length: Full length
• Purity: >95%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl
• Publication:
  A Chemical Biology Approach to Phosphoinositide Metabolic Analysis (2017)
  Required hydrophobicity of fluorescent reporters for phosphatidylinositol family of lipid enzymes (2017)

Gene Information

• Gene Name: PI4K2A phosphatidylinositol 4-kinase type 2 alpha [ Homo sapiens ]
• Official Symbol: PI4K2A
• Synonyms: PI4K2A; phosphatidylinositol 4-kinase type 2 alpha; phosphatidylinositol 4-kinase type 2-alpha; DKFZP761G1923; PI4KII; PIK42A; phosphatidylinositol 4-kinase type II-alpha; phosphatidylinositol 4-kinase type II (PI4KII); RP11-548K23.6; DKFZp761G1923
• Gene ID: 55361
• mRNA Refseq: NM_018425
• Protein Refseq: NP_060895
• MIM: 609763
• UniProt ID: Q9BTU6
• Chromosome Location: 10q24
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Canonical Wnt signaling pathway, organism-specific biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, conserved biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem
• Function: 1-phosphatidylinositol 4-kinase activity; ATP binding; kinase activity; magnesium ion binding; nucleotide binding; transferase activity