Active Recombinant Human SIK3, GST-tagged

• Cat.No.: SIK3-1482H
• Product Overview: Recombinant human SIK3 (1-307) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: SIK3 (QSK) is a serine/threonine-protein kinase, belongs to QIK subfamily. The phosphorylation of SIK3 by LKB1 through the 14-3-3 binding enhances its catalytic activity and leads its localization to punctate structures within the cytoplasm. Overexpression of SIK3 promotes G1/S cell cycle progression with ovarian cancer. There are two sites (H331L and A1103V) were mutated at significant frequency in breast cancer (3). SIK3 is a novel tumor-associated antigen (TAA).
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 140 nmol/min/mg
• Molecular Mass: ~62 kDa
• Protein length: 1-307 aa
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: SIK3 SIK family kinase 3 [ Homo sapiens ]
• Official Symbol: SIK3
• Synonyms: SIK3; SIK family kinase 3; serine/threonine-protein kinase SIK3; FLJ12240; KIAA0999; L19; QSK; salt-inducible kinase 3; serine/threonine-protein kinase QSK; SIK-3
• Gene ID: 23387
• mRNA Refseq: NM_025164
• Protein Refseq: NP_079440
• UniProt ID: Q9Y2K2
• Chromosome Location: 11q23.3
• Pathway: LKB1 signaling events, organism-specific biosystem
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity