Active Recombinant Human STK33, GST-tagged

• Cat.No.: STK33-1489H
• Product Overview: Recombinant full-length human STK33 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: STK33 is a distant member of the CAMK group of serine/threonine kinases but it lacks the calcium/calmodulin-binding domain and the C-terminal regulatory tail. STK33 is highly expressed in testis, lung epithelia, alveolar macrophages, horizontal cells in the retina and in embryonic organs such as heart, brain and spinal cord. STK33 is essential for abnormal cell growth in human cell lines expressing oncogenic mutations in KRAS, but not in human cancer cell lines expressing wildtype KRAS. STK33 is required for survival and proliferation of mutant KRAS-dependent cancer cells, in which it suppresses the S6K1-BAD proapoptotic signaling pathway.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 4.4 nmol/min/mg
• Molecular Mass: ~94 kDa
• Protein length: Full length
• Purity: >80%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: STK33 serine/threonine kinase 33 [ Homo sapiens ]
• Official Symbol: STK33
• Synonyms: STK33; serine/threonine kinase 33; serine/threonine-protein kinase 33
• Gene ID: 65975
• mRNA Refseq: NM_030906
• Protein Refseq: NP_112168
• MIM: 607670
• UniProt ID: Q9BYT3
• Chromosome Location: 11p15.3
• Function: ATP binding; nucleotide binding; protein serine/threonine kinase activity