Active Recombinant Human TLK1, GST-tagged

• Cat.No.: TLK1-1497H
• Product Overview: Recombinant full-length human TLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: Full length
• Description: TLK1 or tousled-like kinase 1 is a nuclear serine/threonine kinase that is potentially involved in the regulation of chromatin assembly. TLK1 is first described in Arabidopsis. TLK1 is a novel target of the DNA damage checkpoint that is rapidly inactivated upon exposure to ionizing radiation (IR) and the inactivation is directly mediated by the S-phase DNA damage checkpoint. The suppression of TLK1 activity after double-strand breaks in the DNA, replication blockade, or low doses of ultraviolet irradiation requires ATM, NBS and CHK1.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 6.2 nmol/min/mg
• Molecular Mass: ~135 kDa
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: TLK1 tousled-like kinase 1 [ Homo sapiens ]
• Official Symbol: TLK1
• Synonyms: TLK1; tousled-like kinase 1; serine/threonine-protein kinase tousled-like 1; KIAA0137; PKU BETA; SNARE protein kinase SNAK; serine threonine protein kinase; PKU-beta
• Gene ID: 9874
• mRNA Refseq: NM_001136554
• Protein Refseq: NP_001130026
• MIM: 608438
• UniProt ID: Q9UKI8
• Chromosome Location: 2q31.1
• Function: ATP binding; nucleotide binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity