Active Recombinant Human TNIK, GST-tagged

• Cat.No.: TNIK-1499H
• Product Overview: Recombinant human TNIK (1-367) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: TNIK or TRAF2 and NCK interacting kinase is characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function. TNIK is mainly expression in brain, heart, and spleen and it is a specific effector of RAP2 which regulate actin cytoskeleton. TNIK is autophosphorylated in a manner dependent upon lys54 in the ATP-binding pocket of its kinase domain and plays a main role in cytoskeleton regulation.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 68 nmol/min/mg
• Molecular Mass: ~67 kDa
• Protein length: 1-367 aa
• Purity: >95%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: TNIK TRAF2 and NCK interacting kinase [ Homo sapiens ]
• Official Symbol: TNIK
• Synonyms: TNIK; TRAF2 and NCK interacting kinase; TRAF2 and NCK-interacting protein kinase; KIAA0551
• Gene ID: 23043
• mRNA Refseq: NM_001161560
• Protein Refseq: NP_001155032
• MIM: 610005
• UniProt ID: Q9UKE5
• Chromosome Location: 3q26.31
• Pathway: Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; TNF receptor signaling pathway, organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; small GTPase regulator activity