Recombinant Human PRKAR1A, His-tagged

• Cat.No.: PRKAR1A-7014H
• Product Overview: Recombinant full-length human PRKAR1A Protein was expressed in Sf9 insect cells using a C-terminal His tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: Full length
• Description: PRKAR1A or the cAMP-dependent protein kinase regulatory subunit type I alpha, is part of the type 1 PKA holoenzyme. PRKAR1A is found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in PRKAR1A gene cause Carney complex (CNC) and PRKAR1A can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40).
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~51 kDa
• Purity: >85%
• Applications: Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.2 μg/μl

Gene Information

• Gene Name: PRKAR1A protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) [ Homo sapiens ]
• Official Symbol: PRKAR1A
• Synonyms: PRKAR1A; protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1); PRKAR1, TSE1; cAMP-dependent protein kinase type I-alpha regulatory subunit; tissue-specific extinguisher 1; protein kinase A type 1a regulatory subunit; cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; MGC17251; DKFZp779L0468
• Gene ID: 5573
• mRNA Refseq: NM_002734
• Protein Refseq: NP_002725
• MIM: 188830
• UniProt ID: P10644
• Chromosome Location: 17q23-q24
• Pathway: Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Aquaporin-mediated transport, organism-specific biosystem; Ca-dependent events, organism-specific biosystem; CaM pathway, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calmodulin induced events, organism-specific biosystem
• Function: cAMP binding; cAMP-dependent protein kinase regulator activity; nucleotide binding; protein binding; ubiquitin protein ligase binding