Recombinant Human RAC3, His-tagged

• Cat.No.: NCOA3-7035H
• Product Overview: Recombinant full-length human RAC3 Protein was expressed in E. coli cells using an N-terminal His tag.

Specification

• Description: RAC3 is a GTPase which belongs to the RHO family of small GTP-binding proteins that regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. RAC3 is primarily expressed in brain and is proposed to play a specific function in neuronal cells. Depletion of RAC3 induces stronger cell adhesion and dramatically increases the outgrowth of neurite-like protrusions whereas overexpression of RAC3 results in a contractile round morphology. RAC3 can exert its function through negatively affecting integrin-mediated cell-matrix adhesions. RAC3 shares with RAC1 the ability to interfere with cadherin-mediated adhesion.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~24 kDa
• Protein length: Full length
• Purity: >95%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.2 μg/μl

Gene Information

• Gene Name: NCOA3 nuclear receptor coactivator 3 [ Homo sapiens ]
• Official Symbol: NCOA3
• Synonyms: NCOA3; nuclear receptor coactivator 3; ACTR; AIB1; bHLHe42; CAGH16; KAT13B; p/CIP; RAC3; receptor associated coactivator 3; SRC 3; SRC3; thyroid hormone receptor activator molecule 1; TNRC16; TRAM 1; CBP-interacting protein; receptor-associated coactivator 3; amplified in breast cancer 1 protein; steroid receptor coactivator protein 3; class E basic helix-loop-helix protein 42; pCIP; AIB-1; CTG26; SRC-3; TNRC14; TRAM-1; MGC141848
• Gene ID: 8202
• mRNA Refseq: NM_001174087
• Protein Refseq: NP_001167558
• MIM: 601937
• UniProt ID: Q9Y6Q9
• Chromosome Location: 20q12
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Repression of Expression by REV-ERBA, organism-specific biosystem; Developmental Biology, organism-specific biosystem; FOXA1 transcription factor network, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem
• Function: androgen receptor binding; chromatin binding; estrogen receptor binding; histone acetyltransferase activity; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; protein N-terminus binding; protein binding; signal transducer activity; thyroid hormone receptor binding; transcription coactivator activity; transcription coactivator activity; transcription coactivator activity; transferase activity, transferring acyl groups