Recombinant Human MPST, His-tagged

• Cat.No.: MPST-7078H
• Product Overview: Recombinant human MPST protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: MPST catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl,20% glycerol, 1mM DTT
• Molecular Mass: 35 kDa (321aa)
• Protein length: 1-297aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASPQL CRALVSAQWV AEALRAPRAG QPLQLLDASW YLPKLGRDAR REFEERHIPG AAFFDIDQCSDRTSPYDHML PGAEHFAEYA GRLGVGAATH VVIYDASDQG LYSAPRVWWM FRAFGHHAVS LLDGGLRHWL RQNLPLSSGK SQPAPAEFRA QLDPAFIKTY EDIKENLESR RFQVVDSRAT GRFRGTEPEP RDGIEPGHIP GTVNIPFTDF LSQEGLEKSP EEIRHLFQEK KVDLSKPLVATCGSGVTACH VALGAYLCGK PDVPIYDGSW VEWYMRARPE DVISEGRGKT H
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: MPST mercaptopyruvate sulfurtransferase [ Homo sapiens ]
• Official Symbol: MPST
• Synonyms: MPST; mercaptopyruvate sulfurtransferase; 3-mercaptopyruvate sulfurtransferase; human liver rhodanese; MST; TST2; MGC24539
• Gene ID: 4357
• mRNA Refseq: NM_001013436
• Protein Refseq: NP_001013454
• MIM: 602496
• UniProt ID: P25325
• Chromosome Location: 22q13.1
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; L-cysteine degradation III, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Sulfur relay system, organism-specific biosystem; Sulfur relay system, conserved biosystem
• Function: 3-mercaptopyruvate sulfurtransferase activity; thiosulfate sulfurtransferase activity; transferase activity