5X Taq PCR Master Mix CMC17 - Creative BioMart

Specification Related Products

Cat. No. :

CMC17

Description :

Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activi and a 5´ flap endonuclease activity. Taq 5X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is best for routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.

Source :

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.

ReagentsSupplied :

MgCl2 (25 mM)

1X Taq Master Mix :

10 mM Tris-HCl (pH 8.6 @ 25°C); 50 mM KCl; 1.5 mM MgCl2; 25 units/ml Taq DNA Polymerase; 0.2 mM dNTPs each; 5% glycerol; 0.08% Ethoxylated tert-octylphenol; 0.05% Tween-20

Applications :

PCR; Primer Extension; Microarray Analysis; High-Throughput PCR

Heat Inactivation :

Unit Definition :

One unit of Taq DNA Polymerase is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Unit AssayConditions :

1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.

ReactionConditions :

1X Taq Master Mix, DNA template and primers in a total reaction volume of 25 or 50 µl.

GeneralNotes :

Taq 5X Master Mix is stable for fifteen freeze-thaw cycles when stored at -20°C. Taq 5X Master Mix is also stable for six months at 4°C, so for frequent use, an aliquot may be kept at 4°C. We recommend assembling all reaction components on ice and quickly transferring the reactions to a thermocycler preheated to the denaturation temperature (95°C).

Reaction Setup :

Component

25 µl Reaction

50 µl Reaction

Final Concentration

10 µM Forward Primer

0.5 µl

1 µl

0.2 µM (0.05–1 µM)

10 µM Reverse Primer

0.5 µl

1 µl

0.2 µM (0.05–1 µM)

Template DNA

variable

<1,000 ng

Taq 5X Master Mix

5 µl

10 µl

Nuclease-free water

to 25 µl

to 50 µl

Step

Temp

Time

Denaturation

95°C	30 seconds

30cycles

95°C	15-30 seconds

45–68°C

15-60 seconds

68°C

1 minute per kb

Final extension

68°C

5 minutes

Hold

4–10°C

Products Types

◆ Recombinant Protein

CMC06

Taq DNA Polymerase

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