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Mouse Anti-Bovine CD2 Hybridoma
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Cat.No. : | CSC-H0181 |
Background : | CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. |
Cell Line Description : | The antibody is directed against Bo CD2, the bovine equivalent of human CD2. It shows species cross-reactivity with goat. |
Depositor : | Dr J Naessens, ILRAD, Nairobi, Kenya |
Immunogen : | Bovine cytotoxic T cells |
Immunological Donor : | BALB/c mouse spleen |
Fusion Species : | Mouse X Mouse Hybridoma |
Myeloma : | P3x63Ag8.653 |
Growth properties : | Suspension |
Propagation : | Complete growth medium: RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C |
Subculturing : | Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C. These cells may require mouse macrophage feeder cells upon resuscitation. |
Mycoplasma : | Mycoplasma Status: Negative (MycoAlert Kit) |
Reactivity : | Anti-CD2, bovine |
Isotype : | IgG2a |
Target : | CD2 |
Application : | Enumeration of bovine lymphocyte populations |
Storage : | Liquid nitrogen |
Safety Considerations : | The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach. |
Ship : | Dry ice |
Gene Name : | CD2 CD2 molecule [ Bos taurus ] |
Official Symbol : | CD2 |
Synonyms : | T-cell surface antigen CD2; CD2 antigen (p50); sheep red blood cell receptor |
Gene ID : | 497022 |
mRNA Refseq : | NM_001011676 |
Protein Refseq : | NP_001011676 |
Chromosome Location : | 3 |
Pathway : | Cell adhesion molecules (CAMs); Cell surface interactions at the vascular wall; Hematopoietic cell lineage; Hemostasis |
Function : | eukaryotic cell surface binding; protein homodimerization activity; receptor activity |
◆ Recombinant Protein
CD2-0737H | Recombinant Human CD2 Protein | +Inquiry |
CD2-1077H | Recombinant Human CD2 Protein (Met1-Asp209), HlgG1 Fc-tagged | +Inquiry |
Cd2-829M | Recombinant Mouse Cd2 Protein, MYC/DDK-tagged | +Inquiry |
CD2-0738H | Recombinant Human CD2 Protein, GST-Tagged | +Inquiry |
CD2-725R | Recombinant Rhesus monkey CD2 Protein, His-tagged | +Inquiry |
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◆ Lysates
CD2-2221MCL | Recombinant Mouse CD2 cell lysate | +Inquiry |
CD2-001CCL | Recombinant Cynomolgus CD2 cell lysate | +Inquiry |
CD2-2553HCL | Recombinant Human CD2 cell lysate | +Inquiry |
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