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| Cat.No.: | CSC-H0181 |
| Background: | CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. |
| Cell Line Description: | The antibody is directed against Bo CD2, the bovine equivalent of human CD2. It shows species cross-reactivity with goat. |
| Depositor: | Dr J Naessens, ILRAD, Nairobi, Kenya |
| Immunogen: | Bovine cytotoxic T cells |
| Immunological Donor: | BALB/c mouse spleen |
| Fusion Species: | Mouse X Mouse Hybridoma |
| Myeloma: | P3x63Ag8.653 |
| Growth properties: | Suspension |
| Propagation: | Complete growth medium: RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C |
| Subculturing: | Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C. These cells may require mouse macrophage feeder cells upon resuscitation. |
| Mycoplasma: | Mycoplasma Status: Negative (MycoAlert Kit) |
| Reactivity: | Anti-CD2, bovine |
| Isotype: | IgG2a |
| Target: | CD2 |
| Application: | Enumeration of bovine lymphocyte populations |
| Storage: | Liquid nitrogen |
| Safety Considerations: | The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship: | Dry ice |
| Gene Name: | CD2 CD2 molecule [ Bos taurus ] |
| Official Symbol: | CD2 |
| Synonyms: | T-cell surface antigen CD2; CD2 antigen (p50); sheep red blood cell receptor |
| Gene ID: | 497022 |
| mRNA Refseq: | NM_001011676 |
| Protein Refseq: | NP_001011676 |
| Chromosome Location: | 3 |
| Pathway: | Cell adhesion molecules (CAMs); Cell surface interactions at the vascular wall; Hematopoietic cell lineage; Hemostasis |
| Function: | eukaryotic cell surface binding; protein homodimerization activity; receptor activity |
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