Mouse Anti-Human p21/WAF1 Monoclonal Antibody

• Cat.No.: CAB-2544MH

Specification

• Cat. No.: CAB-2544MH
• Antigen Description: p21WAF1 is a tumor suppressor gene product critical to maintain proper regulatory control of cell division. p21WAF1 inhibits cyclin/cyclin-dependent kinase (cdk) complexes, causing cell cycle arrest by blocking cell division at the S phase. Various mutations of tumor suppressor genes have been associated with different types of cancers. In these cases, somatic gene therapy with wild-type versions of tumor suppressor genes have been contemplated as anti-cancer therapeutic approaches. p21WAF1 contains p53-binding sites in its promoter and expression of p21WAF1 is induced in WI38 fibroblasts with PMA.
• Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.
• Clone Number: MARL067.
• Host: Mouse.
• Reactivity: Human.
• Physical State: Liquid (sterile filtered)
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
• Preservative: 0.01% (w/v) Sodium Azide.
• Concentration: 1.0 mg/mL (by UV absorbance at 280 nm).
• Application: ELISA: 1:5,000 - 1:20,000; WB: 1:2000 - 1:10000; IP: 1:100; Other: User Optimized.
• Storage: Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Gene Information

• Gene Name: WAF1 cyclin-dependent kinase inhibitor [ Canis lupus familiaris ]
• Official Symbol: WAF1
• Synonyms: cyclin-dependent kinase inhibitor 1A (p21, Cip1); MDA-6; CIP1; CDK-interaction protein 1; wild-type p53-activated fragment 1; SDI1; cyclin-dependent kinase inhibitor 1; CAP20; melanoma differentiation associated protein 6; CDKN1; OTTHUMP00000016298; P21; DNA synthesis inhibitor; p21Cip1/Waf1; MDA6; p21CIP1; p21; Melanoma differentiation-associated protein 6; PIC1; CDK-interacting protein 1
• Gene ID: 403706
• mRNA Refseq: AF076469
• Protein Refseq: AAC27627
• MIM: 116899
• UniProt ID: P38936
• Chromosome Location: 6p21
• Function: proliferation in response to DNA damage; binds to and inhibits cyclin-dependent kinase activity; preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression