||Recombinant e.colirnhA protein, fused to His-tag at N-terminus, was expressed in E.coliand purified by using conventional chromatography techniques.
||rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5"-phosphomonoester and is able to bind magnesium or manganese as cofactors.
||Liquid. In 20mM Tris-HClbuffer(pH 8.0) containing 10% glycerol, 2mM DTT.
||20 kDa (178aa), confirmed by MALDI-TOF
||> 95 % by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSMLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.