||Recombinant Human FSHB was produced in human cell transiently transfected with two expression plasmids encoding the human FSHα (Accession # P01215) (Met1-Ser116) and human FSHβ (Accession # P01225) (Met1-Glu129).
||Human Follicle-stimulating hormone (FSH) is a member of glycoprotein hormones subunit beta family, which also includes LH, chorionic gonadotropin (CG) and thyroid-stimulating hormone (TSH). FSH and its family members are heterodimers consisting of non-covalently linked α- and β-subunits. They share an identical α subunit, and β-subunits vary. FSH has a unique β-subunit (FSHβ), which confers its specific biologic activity and is responsible for interaction with the FSH-receptor which belongs to a subfamily of GPCRs called leucine-rich-repeat-containing GPCRs (LGRs). FSH is secreted from the pituitary gland and regulates reproduction in mammals. FSH stimulates sertoli cell proliferation in testes and supports spermatogenesis in males, and induces the maturation of ovarian follicles in females.
||The ED50 of 80~450 pg/ml is measured by cAMP accumulation in human FSH Receptor transfected Chinese Hamster Ovary cells.
||Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
||Predicted N terminal amino acids are Ala25 (α subunit), Cys21 (β subunit).
||Less than 0.1ng/µg (1 IEU/µg) determined by LAL test.
||The protein was lyophilized after extensive dialysis against PBS.
||It is recommended to reconstitute the lyophilized rHuFSH in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
||Lyophilized rHuFSH should be stored desiccated below -18°C upon receipt. Upon reconstitution rHuFSH should be stored at 4°C, which is stable for 2 weeks. For long-term storage, aliquots of reconstituted samples are stable for 3 months below -80°C. Please avoid freeze-thaw cycles.