||Recombinant human CHMP2B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-213 aa).
||CHMP2B belongs to the vacuolar sorting protein family. It is a component of the ESCRT-III complex. This complex is crucial for sorting endosomal articles into multivesicular bodies (MVBs), and are also required for the formation of these bodies. CHMP2B widely expressed in brain, heart, skeletal muscle, small intestine, pancreas, lung, placenta and leukocytes.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
||26.1 kDa (233aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)
||> 90 % by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MASLFKKKTV DDVIKEQNRE LRGTQRAIIR DRAALEKQEK QLELEIKKMA KIGNKEACKV LAKQLVHLRK QKTRTFAVSS KVTSMSTQTK VMNSQMKMAG AMSTTAKTMQ AVNKKMDPQK TLQTMQNFQK ENMKMEMTEE MINDTLDDIF DGSDDEEESQ DIVNQVLDEI GIEISGKMAK APSAARSLPS ASTSKATISD EEIERQLKAL GVD
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.