"DNASE1" Related Products

Expand
Expand
Expand

DNase I

Cat. No.: CMC04
Product Overview: DNase I is a glycosylated polypeptide that is commonly used to degrade unwanted single- and double-stranded DNA into 5´phosphodinucleotide and oligonucleotide fragments. The properties of DNase I can be modified by divalent ions. For example, in the presence of Mg2+, Ca2+ or Zn2+, DNase I degrades DNA by making random single-strand nicks in the phosphate backbone. In the presence of divalent transition metals such as Mn2+ or Co2+, DNase I creates double-strand breaks, resulting in fragments with 0-2 nucleotide overhangs. DNase I is suitable for removal of genomic DNA from cell lysates, removal of plasmid from in vitro transcribed RNA, nick translation and DNase I footprinting.
Source: E.coli
Packaging: DNase I(RNase Free)(5U/ul) 1000U, 200ul, 2×DNase Buffer, 1000ul.
Concentration: 5U/ul
Unit Definition: One unit is that amount of enzyme causing an increase in absorbance at 260 nm by 0.001 per minute at 25°C.
Reaction buffer: [2×DNase Buffer ] 200mM Tris-HCl (pH7.5, 20mM CaCl2, 20mM MgCl2.
Storage buffer: [pH5.3]75mM NaCl, 10mM NaAc, 50% glycerol(v/v)
Application: RT-PCR; In vitro transcription; Nick translation; Shot gun sequencing; Foot printing.
Storage: -20°C.

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.