DNase I

• Cat.No.: CMC04
• Product Overview: DNase I is a glycosylated polypeptide that is commonly used to degrade unwanted single- and double-stranded DNA into 5´phosphodinucleotide and oligonucleotide fragments. The properties of DNase I can be modified by divalent ions. For example, in the presence of Mg2+, Ca2+ or Zn2+, DNase I degrades DNA by making random single-strand nicks in the phosphate backbone. In the presence of divalent transition metals such as Mn2+ or Co2+, DNase I creates double-strand breaks, resulting in fragments with 0-2 nucleotide overhangs. DNase I is suitable for removal of genomic DNA from cell lysates, removal of plasmid from in vitro transcribed RNA, nick translation and DNase I footprinting.

Specification

• Cat. No.: CMC04
• Source: E.coli
• Packaging: DNase I(RNase Free)(5U/ul) 1000U, 200ul, 2×DNase Buffer, 1000ul.
• Concentration: 5U/ul
• Unit Definition: One unit is that amount of enzyme causing an increase in absorbance at 260 nm by 0.001 per minute at 25°C.
• Reaction buffer: [2×DNase Buffer ] 200mM Tris-HCl (pH7.5, 20mM CaCl2, 20mM MgCl2.
• Storage buffer: [pH5.3]75mM NaCl, 10mM NaAc, 50% glycerol(v/v)
• Application: RT-PCR; In vitro transcription; Nick translation; Shot gun sequencing; Foot printing.
• Storage: -20°C.

Reviews

12/06/2022

Dependable antibody validation, vital for our immunoprecipitation studies.

01/10/2022

Top-quality protein purification service, essential for our lab's work.

02/22/2019

Fast and reliable protein expression, a critical part of our projects.

Q&As

How does DNASE1 (Deoxyribonuclease I) contribute to DNA degradation and clearance, particularly in apoptotic cells?

DNASE1 helps degrade DNA in apoptotic cells, ensuring proper clearance and preventing inflammation.

How does DNASE1 deficiency lead to the development of systemic lupus erythematosus (SLE) and other autoimmune diseases?

DNASE1 deficiency can contribute to autoimmune diseases like SLE by allowing self-DNA accumulation.

What is the impact of DNASE1 on the regulation of inflammation and immune system activation?

DNASE1 regulates inflammation and immune responses by controlling extracellular DNA levels.

How does DNASE1 influence the resolution of inflammation and tissue repair processes?

DNASE1 aids in resolving inflammation and promoting tissue repair by degrading DNA in damaged tissues.

What are the mechanisms by which DNASE1 modulates the stability and integrity of extracellular DNA?

DNASE1 maintains the stability and integrity of extracellular DNA, preventing inappropriate immune activation.

How do alterations in DNASE1 expression or activity affect susceptibility to chronic inflammatory conditions?

Changes in DNASE1 function can increase susceptibility to chronic inflammation and autoimmune disorders.

What role does DNASE1 play in preventing the accumulation of self-DNA and autoimmune responses?

DNASE1 prevents the accumulation of self-DNA, reducing the risk of autoimmune reactions.