||DNase I is a glycosylated polypeptide that is commonly used to degrade unwanted single- and double-stranded DNA into 5´phosphodinucleotide and oligonucleotide fragments. The properties of DNase I can be modified by divalent ions. For example, in the presence of Mg2+, Ca2+ or Zn2+, DNase I degrades DNA by making random single-strand nicks in the phosphate backbone. In the presence of divalent transition metals such as Mn2+ or Co2+, DNase I creates double-strand breaks, resulting in fragments with 0-2 nucleotide overhangs. DNase I is suitable for removal of genomic DNA from cell lysates, removal of plasmid from in vitro transcribed RNA, nick translation and DNase I footprinting.
||DNase I(RNase Free)(5U/ul) 1000U, 200ul, 2×DNase Buffer, 1000ul.
||One unit is that amount of enzyme causing an increase in absorbance at 260 nm by 0.001 per minute at 25°C.
||[2×DNase Buffer ] 200mM Tris-HCl (pH7.5, 20mM CaCl2, 20mM MgCl2.
||[pH5.3]75mM NaCl, 10mM NaAc, 50% glycerol(v/v)
||RT-PCR; In vitro transcription; Nick translation; Shot gun sequencing; Foot printing.