DNase I

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DNase I

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Cat. No. : CMC04
Product Overview : DNase I is a glycosylated polypeptide that is commonly used to degrade unwanted single- and double-stranded DNA into 5´phosphodinucleotide and oligonucleotide fragments. The properties of DNase I can be modified by divalent ions. For example, in the presence of Mg2+, Ca2+ or Zn2+, DNase I degrades DNA by making random single-strand nicks in the phosphate backbone. In the presence of divalent transition metals such as Mn2+ or Co2+, DNase I creates double-strand breaks, resulting in fragments with 0-2 nucleotide overhangs. DNase I is suitable for removal of genomic DNA from cell lysates, removal of plasmid from in vitro transcribed RNA, nick translation and DNase I footprinting.
Source : E.coli
Packaging : DNase I(RNase Free)(5U/ul) 1000U, 200ul, 2×DNase Buffer, 1000ul.
Concentration : 5U/ul
Unit Definition : One unit is that amount of enzyme causing an increase in absorbance at 260 nm by 0.001 per minute at 25°C.
Reaction buffer : [2×DNase Buffer ] 200mM Tris-HCl (pH7.5, 20mM CaCl2, 20mM MgCl2.
Storage buffer : [pH5.3]75mM NaCl, 10mM NaAc, 50% glycerol(v/v)
Application : RT-PCR; In vitro transcription; Nick translation; Shot gun sequencing; Foot printing.
Storage : -20°C.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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