Proteinase K

• Cat.No.: CMC05

Specification

• Cat. No.: CMC05
• Description: Proteinase K (also protease K or endopeptidase K) EC 3.4.21.64 is a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album). Proteinase K is able to digest native keratin (hair), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification. It is highly-suited to this application since the enzyme is active in the presence of chemicals that denature proteins, such as SDS and urea, chelating agents such as EDTA, sulfhydryl reagents, as well as trypsin or chymotrypsin inhibitors. Proteinase K is also stable over a wide pH range (4-12), with a pH optimum of pH 7.5-12.
• Source: Tritirachium album limber
• Molecular Weight: 29,730Da
• Form: Contains 100mg Proteinase K, Lyophilized Powder
• Specific Activity: 40U/mg protein, 17.5U/mg dry weight
• DNase Activity: none detectable enzyme activity with λ DNA after 6 hours incubation at 37ºC.
• RNase Activity: none detectable of ribnuclease activity after 16hours incubation at 25 º C
• Unit Definitions: one unit is defined as the amount of enzyme that will liberate 1 μmol of tyrosine per minute at 37º C, pH7.5
• Applications: Proteinase K is a broad substrate non-specific serine Proteinase. It is very stable at pH 4-12. It was used on isolating mRNA, genomic DNA and digesting unwanted proteins during DNA and RNA preparations from different kinds of cells. It’s been used on glycoprotein modification and protein structure studies also. Proteinase K is active with SDS, urea and EDTA.
• Storage Conditions: Store vial at 2-4ºC upon receipt. Dissolve and aliquot and store at -20ºC recommended. Expiration date is one year of powder and six months of sterile liquid at -20 ºC to 4ºC. Use sterile filtration for liquid enzyme storage if keep it at 2-8ºC over a week.
• Storage Buffer: 20mM Tris-HCL (pH7.4), 1mM CaCl2, 50% Glycerol. (Dilution buffer without glycerol)
• Publication:
  Population genetics of the collector urchin, Tripneustes gratilla, in the Indonesian archipelago (2019)
  Population genetic structure in the coral reef associated ascidian, Polycarpa aurata, throughout Wallacea, Indonesia (2020)