||Recombinant human CERT protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 33.1 kDa (290aa).
||CERT is a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
||> 95% by SDS – PAGE.
||0.5 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMLH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL AD NA I IIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VR AKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAV AK R EYPK FLKRFTSYVQ EKTAGKPILF.
||can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.