Recombinant Human RAP2B, His-tagged

• Cat.No.: RAP2B-7522H
• Product Overview: Recombinant human RAP2B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Antigen Description: Ras-related protein Rap-2b, also known as RAP2B, belongs to a family of RAS-related genes. Unlike normal ras proteins that have a nontransforming glutamine residue at aa 61, Rap2B has a threonine residue in that position. This change makes the intrinsic GTPase activity for Rap2B lower, thereby allowing it to exist in the activated state for a longer period of time than normal ras proteins. RAP2B is a platelet protein that is activated by thrombin and involved in platelet activation. RAP2B may be a novel candidate oncogene that plays important roles in carcinogenesis through activation of NF-kappaB pathway.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 22.9kDa (206aa) confirmed by MALDI-TOF
• Protein length: 1-183aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMREYKVV VLGSGGVGKS ALTVQFVTGS FIEKYDPTIE DFYRKEIEVD SSPSVLEILD TAGTEQFASM RDLYIKNGQG FILVYSLVNQ QSFQDIKPMR DQIIRVKRYE RVPMILVGNK VDLEGEREVS YGEGKALAEE WSCPFMETSA KNKASVDELF AEIVRQMNYA AQPNGDEGCC SACVIL
• Purity: >95% as determined by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: RAP2B RAP2B, member of RAS oncogene family [ Homo sapiens ]
• Official Symbol: RAP2B
• Synonyms: RAP2B; RAP2B, member of RAS oncogene family; ras-related protein Rap-2b; Ras family small GTP binding protein RAP2B; Ras related protein RAP 2B; small GTP binding protein; MGC20484
• Gene ID: 5912
• mRNA Refseq: NM_002886
• Protein Refseq: NP_002877
• MIM: 179541
• UniProt ID: P61225
• Chromosome Location: 3q25.2
• Function: GTP binding; GTPase activity; nucleotide binding; protein domain specific binding