||Recombinant streptococcal protein G is produced in Escherichia coli using sequence from Streptococcus C1-C2-C3.
||Recombinant streptococcal protein G lacking the albumin binding region thereby avoiding undesirable reactions with albumin, though the Fc binding domain is still present.
||The Protein G contains amino acids 190-384 having a molecular mass of 21.6 kDa. The Protein-G migrates on SDS-PAGE around 32 kDa.
||Sterile Filtered White lyophilized (freeze-dried) powder.
||Lyophilized white Powder containing no additives.
||>95% as determined by SDS-PAGE and HPLC.
|Amino Acid Sequence:
||MTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDAT KTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTEAVDAATAEKVFK QYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTL KGETTTKAVDAETAEKAFKQYANDNGVDGVWTYDDATKTFTVTE.
||a. Binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a. b. Does not bind to human IgM, IgD and IgA.
||Reconstitution with deionized water or PBS.
||After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
||Protein G binds to the constant region of many species of immunoglobulin G. It can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity.
|Introduction||Streptococcus is a genus of spherical Gram-positive bacteria belonging to the phylum Firmicutes and the lactic acid bacteria group. Certain Streptococcus species are responsible for many cases of meningitis, bacterial pneumonia, endocarditis, erysipelas and necrotizing fasciitis (the ''flesh-eating'' bacterial infections). However, many streptococcal species are nonpathogenic, and form part of the commensal human microbiome of the mouth, skin, intestine, and upper respiratory tract. Furthermore, streptococci are a necessary ingredient in producing Emmentaler ("Swiss") cheese.|
|Keywords||RPG; Protein G; ProteinG; IgG binding protein G; IgG-binding protein G;Immunoglobulin G binding protein G [Precursor]; spg|