||Recombinant human ACADL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||ACADL belongs to the acyl-CoA dehydrogenase family, existing as a homotetramer. It is involved in the catabolism of fatty acids and amino acids and they provide a major source of energy for the heart and skeletal muscle. Defect in the gene encoding ACADL leads to non-ketotic hypoglycemia and hypotonia, muscle weakness.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 30% glycerol, 0.1 M NaCl.
||46.7 kDa (421aa), confirmed by MALDI-TOF
||> 85 % by SDS-PAGE
||1 mg/ml (determined by Bradford)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGGEERLETP SAKKLTDIGI RRIFSPEHDI FRKSVRKFFQ EEVIPHHSEW EKAGEVSREV WEKAGKQGLL GVNIAEHLGG IGGDLYSAAI VWEEQAYSNC SGPGFSIHSG IVMSYITNHG SEEQIKHFIP QMTAGKCIGA IAMTEPGAGS DLQGIKTNAK KDGSDWILNG SKVFISNGSL SDVVIVVAVT NHEAPSPAHG ISLFLVENGM KGFIKGRKLH KMGLKAQDTA ELFFEDIRLP ASALLGEENK GFYYIMKELP QERLLIADVA ISASEFMFEE TRNYVKQRKA FGKTVAHLQT VQHKLAELKT HICVTRAFVD NCLQLHEAKR LDSATACMAK YWASELQNSV AYDCVQLHGG WGYMWEYPIA KAYVDARVQP IYGGTNEIMK ELIAREIVFD K
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA; Fatty acid metabolism; Fatty acid, triacylglycerol, and ketone body metabolism; Metabolic pathways; Metabolism of lipids and lipoproteins; Mitochondrial Fatty Acid Beta-Oxidation; PPAR signaling pathway; mitochondrial fatty acid beta-oxidation of saturated fatty acids