Recombinant Human APP, His & GST-tagged

• Cat.No.: APP-3850H
• Product Overview: Recombinant Human APP (Asp672-Ala713), fused with the N-terminal polyhistidine-tagged GST tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Lyophilized from sterile PBS, 10% glycerol, pH 7.4
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human Beta-APP42/GST chimera consists of 280 amino acids and has a calculated molecular mass of 32.4 KDa. It migrates as an approximately 34 KDa band in SDS-PAGE under reducing conditions.
• Protein length: Asp672-Ala713
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: APP amyloid beta (A4) precursor protein [ Homo sapiens ]
• Official Symbol: APP
• Gene ID: 351
• mRNA Refseq: NM_000484
• Protein Refseq: NP_000475
• MIM: 104760
• UniProt ID: P05067