Active Recombinant Human BMPR1A protein, His-tagged

• Cat.No.: BMPR1A-889H
• Product Overview: Active Recombinant Human BMPR1A protein(Met 1-Arg 152)(NP_004320.2) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-152 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit recombinant human BMP4 induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.2-1 μg/mL in the presence of 50 ng/ml of recombinant human BMP4.
• Molecular Mass: The secreted recombinant human ALK3 consists of 141 amino acids and has a predicted molecular mass of 15.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhALK3 is approximately 28-33 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BMPR1A bone morphogenetic protein receptor, type IA [ Homo sapiens ]
• Official Symbol: BMPR1A
• Synonyms: BMPR1A; bone morphogenetic protein receptor, type IA; ACVRLK3; bone morphogenetic protein receptor type-1A; ALK3; CD292; ALK-3; BMPR-1A; BMP type-1A receptor; activin receptor-like kinase 3; activin A receptor, type II-like kinase 3; serine/threonine-protein kinase receptor R5; SKR5; 10q23del
• Gene ID: 657
• mRNA Refseq: NM_004329
• Protein Refseq: NP_004320
• MIM: 601299
• UniProt ID: P36894