Active Recombinant Human CA5B protein(Cys34-Pro317), His-tagged

• Cat.No.: CA5B-1125H
• Product Overview: Recombinant Human CA5B (Q9Y2D0) (Cys 34-Pro 317) was expressed in E. coli, fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: C-His
• Protein length: Cys34-Pro317
• Form: Lyophilized from sterile 20mM Tris, 50mM NaCl, 0.05% Brij-35, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its esterase activity. The specific activity is >150 pmoles/min/μg.
• Molecular Mass: The recombinant human CA5B comprises 295 amino acids and migrates as an approximately 34 kDa band as predicted in SDS-PAGE under reducing conditions.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CA5B carbonic anhydrase VB, mitochondrial [ Homo sapiens ]
• Official Symbol: CA5B
• Synonyms: CA5B; carbonic anhydrase VB, mitochondrial; carbonic anhydrase 5B, mitochondrial; carbonic dehydratase; carbonate dehydratase VB; CA-VB; MGC39962
• Gene ID: 11238
• mRNA Refseq: NM_007220
• Protein Refseq: NP_009151
• MIM: 300230
• UniProt ID: Q9Y2D0