Active Recombinant Human Chemokine (C-C Motif) Ligand 18 (Pulmonary And Activation-Regulated)

• Cat.No.: CCL18-300H
• Product Overview: Recombinant Human Macrophage Inflammatory Protein-4 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7813 Daltons.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Chemokine (C-C motif) ligand 18 (CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine).
• Form: Lyopohilized with no additives.
• Purity: Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: Human Macrophage Inflammatory Protein-4 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human T lymphocytes at 1.0-8.0 ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg"s/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: AQVGTNKELC CLVYTSWQIP QKFLVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Gene Information

• Gene Name: CCL18 chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated) [ Homo sapiens ]
• Official Symbol: CCL18
• Synonyms: CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18; CCL18_HUMAN; C-C motif chemokine 18 [Precursor]; Small-inducible cytokine A18;Macrophage inflammatory protein 4; ulmonary and activation-regulated chemokine;CC chemokine PARC;Alternative macrophage activation-associated CC chemokine 1; Dendritic cell chemokine 1; CCL18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
• Gene ID: 6362
• mRNA Refseq: NM_002988
• Protein Refseq: NP_002979
• MIM: 603757
• UniProt ID: P55774
• Chromosome Location: 17q11.2
• Pathway: chemokine activity
• Function: Cytokine-cytokine receptor interaction