Recombinant Human CD276 protein, His-tagged CD276-3884H

Recombinant Human CD276 protein, His-tagged

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Recombinant Human CD276 protein, His-tagged

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Cat.No. : CD276-3884H
Product Overview : Recombinant Human CD276 (Gly27-Thr461) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Gly27
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 48.5 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gly27-Thr461
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CD276-3884H :

Human B7-H3 (4Ig), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of CD276-3884H :

Immobilized Human B7-H3 (4Ig), His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

Bioactivity-ELISA :

Immobilized Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Human B7-H3 (4Ig), His Tag with a linear range of 1-10 ng/mL (Routinely tested).

Bioactivity-Cell based assay of CD276-3884H :

Human B7-H3 (4Ig), His Tag inhibits Anti-CD3-induced proliferation of PBMC. The EC50 for this effect is 7.95 μg/mL (Routinely tested).

Gene Name : CD276
Official Symbol : CD276
Synonyms : CD276; CD276 molecule; CD276 antigen; B7 H3; B7H3; B7RP 2; B7 homolog 3; costimulatory molecule; B7-H3; B7RP-2; 4Ig-B7-H3
Gene ID : 80381
mRNA Refseq : NM_001024736
Protein Refseq : NP_001019907
MIM : 605715
UniProt ID : Q5ZPR3

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