CD276

  • Official Full Name

    CD276 molecule

  • Overview

    B7-H3 is a member of the B7 gene family. It has 88% amino acid sequence identity with mouse B7-H3. It is mostly expressed on professional APCs, including B cells, macrophages, and dendritic cells. Its expression on dendritic cells appears to be up-regulat
  • Synonyms

    CD276; CD276 molecule; CD276 antigen; B7 H3; B7H3; B7RP 2; B7 homolog 3; costimulatory molecule; B7-H3; B7RP-2; 4Ig-B7-H3;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • GMP Proteins
  • Protein Pre-coupled Magnetic Beads
  • Chromatography Reagents
  • Chicken
  • Cynomolgus
  • Cynomolgus Monkey
  • Human
  • Monkey
  • Mouse
  • Rat
  • Rhesus monkey
  • Zebrafish
  • CHO
  • CHO cells
  • E.coli
  • HEK293
  • HEK293 cells
  • HEK293T
  • Human Cell
  • Human Cells
  • Mammalian Cell
  • Mammalian cells
  • Avi|Fc
  • C
  • Avi
  • His
  • hFc
  • DDK|His
  • DYKDDDDK
  • Fc
  • GST
  • HIgG1 Fc
  • hIgG4
  • His (Fc)
  • His(C
  • ter)
  • His\Avi
  • His|Flag|StrepII
  • human|IgG1|Fc
  • mFc
  • MIgG2a Fc
  • Myc
  • DDK
  • MYC
  • Myc|DDK
  • N/A
  • Tag Free
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human CD276-757H Active Recombinant Human CD276 Protein, His-tagged Mammalian cells His Leu29-Pro245
Human CD276-2002H Active Recombinant Human CD276, HIgG1 Fc-tagged CHO Fc
Human CD276-542H Active Recombinant Human CD276, Fc-tagged Human Cell Fc
Human CD276-2001H Active Recombinant Human CD276, MIgG2a Fc-tagged CHO Fc
Human CD276-756H Active Recombinant Human CD276 Protein, Fc Chimera Mammalian cells Fc
Human CD276-543H Active Recombinant Human CD276, Fc-tagged, Biotinylated Human Cell Fc
Human CD276-2002HAF555 Active Recombinant Human CD276 Protein, hFc-tagged, Alexa Fluor 555 conjugated CHO hFc
Human CD276-756HAF555 Active Recombinant Human CD276 Protein, Fc-tagged, Alexa Fluor 555 conjugated Mammalian cells Fc
Human CD276-1286H Active Recombinant Human CD276 protein, His-Avi-tagged, Biotinylated HEK293 His-Avi Leu 29 - Pro 245
Human CD276-053H Active Recombinant Human CD276 protein, Fc/Avi-tagged, Biotinylated CHO cells Fc/Avi Leu29-Pro245
Human CD276-2002HAF647 Active Recombinant Human CD276 Protein, hFc-tagged, Alexa Fluor 647 conjugated CHO hFc
Human CD276-756HAF647 Active Recombinant Human CD276 Protein, Fc-tagged, Alexa Fluor 647 conjugated Mammalian cells Fc
Human CD276-051H Active Recombinant Human CD276 protein, Fc/Avi-tagged, Biotinylated CHO cells Fc/Avi Leu29-Thr461
Human CD276-2001HAF488 Active Recombinant Human CD276 Protein, MIgG2a mFc-tagged, Alexa Fluor 488 conjugated CHO mFc
Human CD276-2002HF Active Recombinant Human CD276 Protein, hFc-tagged, FITC conjugated CHO hFc
Human CD276-542HF Active Recombinant Human CD276 Protein, Fc-tagged, FITC conjugated HEK293 Fc
Human CD276-056H Active Recombinant Human CD276 protein, His/Avi-tagged, Biotinylated HEK293 cells His/Avi Leu29-Thr461
Human CD276-542HAF488 Active Recombinant Human CD276 Protein, Fc-tagged, Alexa Fluor 488 conjugated HEK293 Fc
Human CD276-2002HAF488 Active Recombinant Human CD276 Protein, hFc-tagged, Alexa Fluor 488 conjugated CHO hFc
Human CD276-756HF Active Recombinant Human CD276 Protein, Fc-tagged, FITC conjugated Mammalian cells Fc
Human CD276-756HAF488 Active Recombinant Human CD276 Protein, Fc-tagged, Alexa Fluor 488 conjugated Mammalian cells Fc
Human CD276-2001HAF647 Active Recombinant Human CD276 Protein, MIgG2a mFc-tagged, Alexa Fluor 647 conjugated CHO mFc
Human CD276-2001HAF555 Active Recombinant Human CD276 Protein, MIgG2a mFc-tagged, Alexa Fluor 555 conjugated CHO mFc
Human CD276-2906H Active Recombinant Human CD276 protein, Fc-tagged HEK293 Fc Leu 29-Pro 245
Human CD276-542HAF555 Active Recombinant Human CD276 Protein, Fc-tagged, Alexa Fluor 555 conjugated HEK293 Fc
Human CD276-542HAF647 Active Recombinant Human CD276 Protein, Fc-tagged, Alexa Fluor 647 conjugated HEK293 Fc
Human CD276-2001HF Active Recombinant Human CD276 Protein, MIgG2a mFc-tagged, FITC conjugated CHO mFc
Human CD276-2901H Active Recombinant Human CD276 protein, His-tagged, FITC-Labeled HEK293 His Gly 27-Thr 461
Human CD276-2900H Active Recombinant Human CD276 protein, Fc-tagged, FITC-Labeled HEK293 Fc Gly 27-Thr 461
Human CD276-2902H Active Recombinant Human CD276 protein, Fc-tagged HEK293 Fc Gly 27-Thr 461
Human CD276-303H Recombinant Human CD276 protein, Fc-tagged HEK293 human/IgG1/Fc Gly27-Thr461
Human CD276-7674H Recombinant Human CD276 protein, His-tagged E.coli His Thr47~Ser219
Human CD276-0766H Recombinant Human CD276 Protein, His-Flag-StrepII-Tagged Human Cell His/Flag/StrepII
Human CD276-002H Recombinant Human CD276 Protein, DDK/His-tagged HEK293 DDK/His
Human CD276-4893H Recombinant Human CD276 protein, His-tagged, biotinylated HEK293 His Met1-Thr461
Human CD276-10935H Recombinant Human CD276, GST-tagged E.coli GST 41-181a.a.
Human CD276-183H Recombinant Human CD276, Fc-tagged CHO Fc
Human CD276-3884H Recombinant Human CD276 protein, His-tagged HEK293 His Met1-Thr461
Human CD276-1959HCL Recombinant Human CD276 cell lysate Human Cell N/A
Human CD276-1287H Recombinant Human CD276 Protein, Fc-Avi-tagged, Biotinylated HEK293 Avi/Fc
Human CD276-756HP Recombinant Human CD276 protein, Fc-tagged, R-PE labeled HEK293 Fc Met1-Thr461
Human CD276-183HAF488 Recombinant Human CD276 Protein, Fc-tagged, Alexa Fluor 488 conjugated CHO Fc
Human CD276-156H Recombinant Human CD276 Protein, DYKDDDDK-tagged Human Cells DYKDDDDK
Human CD276-127H Recombinant Human CD276 protein, His-Avi-tagged HEK293 His-Avi It contains Gly27 - Thr461.
Human CD276-183HA Recombinant Human CD276 protein, Fc-tagged, APC labeled CHO Fc
Human CD276-2001HA Recombinant Human CD276 protein, MIgG2a Fc-tagged, APC labeled CHO MIgG2a Fc
Human CD276-303HAF488 Recombinant Human CD276 Protein, hFc-tagged, Alexa Fluor 488 conjugated HEK293 hFc Gly27-Thr461
Human CD276-586H Recombinant Human CD276 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human CD276-2002HA Recombinant Human CD276 protein, HIgG1 Fc-tagged, APC labeled CHO HIgG1 Fc
Human CD276-303HA Recombinant Human CD276 protein, Fc-tagged, APC labeled HEK293 Fc
Human CD276-3884HAF488 Recombinant Human CD276 Protein, His-tagged, Alexa Fluor 488 conjugated HEK293 His Gly27-Thr461
Human CD276-303HAF555 Recombinant Human CD276 Protein, hFc-tagged, Alexa Fluor 555 conjugated HEK293 hFc Gly27-Thr461
Human CD276-347H Recombinant Human CD276 protein, Fc-tagged HEK293 human/IgG1/Fc 217
Human CD276-348H Recombinant Human CD276 protein, His-tagged HEK293 His 217
Human CD276-3054H Recombinant Human CD276 Protein, MYC/DDK-tagged HEK293 Myc/DDK
Human CD276-3884HAF555 Recombinant Human CD276 Protein, His-tagged, Alexa Fluor 555 conjugated HEK293 His Gly27-Thr461
Human CD276-127HG Active GMP Recombinant Human CD276 protein HEK293 DNA sequence encoding Human B7-H3(UniProtKB/Swiss-Prot: Q5ZPR3.1) was expressed with His tag at the C-terminal.
Human CD276-502H Recombinant Human CD276 Protein (ECD) (Met1-Thr461), HIgG1 Fc-tagged, Biotinylated HEK293 HIgG1 Fc Met1-Thr461
Human CD276-183HP Recombinant Human CD276 protein, Fc-tagged, R-PE labeled CHO Fc
Human CD276-303HAF647 Recombinant Human CD276 Protein, hFc-tagged, Alexa Fluor 647 conjugated HEK293 hFc Gly27-Thr461
Human CD276-1238H Recombinant Human CD276 protein(Met1-Thr243), His-tagged HEK293 C-His Met1-Thr243
Human CD276-126HB Recombinant Human CD276 protein, His-Avi-tagged, Biotinylated HEK293 His-Avi It contains Gly27 - Thr461.
Human CD276-2001HP Recombinant Human CD276 protein, MIgG2a Fc-tagged, R-PE labeled CHO MIgG2a Fc
Human CD276-3884HAF647 Recombinant Human CD276 Protein, His-tagged, Alexa Fluor 647 conjugated HEK293 His Gly27-Thr461
Human CD276-1363H Recombinant Human CD276 protein(Met1-Thr461), hFc-tagged, Biotinylated HEK293 C-hFc Met1-Thr461
Human CD276-155H Recombinant Human CD276 Protein, DYKDDDDK-tagged Human Cells DYKDDDDK
Human CD276-2002HP Recombinant Human CD276 protein, HIgG1 Fc-tagged, R-PE labeled CHO HIgG1 Fc
Human CD276-303HF Recombinant Human CD276 Protein, hFc-tagged, FITC conjugated HEK293 hFc Gly27-Thr461
Human CD276-599H Recombinant Human CD276 Protein HEK293 534
Human CD276-600H Recombinant Human CD276 Protein, Fc-tagged HEK293 Fc 534
Human CD276-303HP Recombinant Human CD276 protein, Fc-tagged, R-PE labeled HEK293 Fc Met1-Thr461
Human CD276-3884HF Recombinant Human CD276 Protein, His-tagged, FITC conjugated HEK293 His Gly27-Thr461
Human CD276-2631H-B Recombinant Human CD276 Protein Pre-coupled Magnetic Beads HEK293
Human CD276-183HAF647 Recombinant Human CD276 Protein, Fc-tagged, Alexa Fluor 647 conjugated CHO Fc
Human CD276-542HP Recombinant Human CD276 protein, Fc-tagged, R-PE labeled HEK293 Fc
Human CD276-27H Recombinant Human CD276 Protein (ECD), His-tagged(C-ter) HEK293 His(C-ter) Leu29-Pro245
Human CD276-183HF Recombinant Human CD276 Protein, Fc-tagged, FITC conjugated CHO Fc
Human CD276-145H Recombinant Human CD276 Protein, C-His-tagged E.coli C-His
Human CD276-542HA Recombinant Human CD276 protein, Fc-tagged, APC labeled HEK293 Fc
Human CD276-2631H Recombinant Human CD276 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human CD276-183HAF555 Recombinant Human CD276 Protein, Fc-tagged, Alexa Fluor 555 conjugated CHO Fc
Human CD276-0987M Active CD276 protein-coupled magnetic Beads HEK293 Gly 27 - Thr 461
Human CD276-756HA Recombinant Human CD276 protein, Fc-tagged, APC labeled Mammalian cells Fc
Human CD276-2645H Recombinant Human CD276 Protein (Met1-Thr461), C-His tagged Mammalian cells C-His Met1-Thr461
Human CD276-02H Recombinant Human CD276 Protein, C-Avi-His tagged, Biotinylated Human Cell C-Avi-His leu29-pro245
Human CD276-174H Recombinant Human CD276 Protein, His\Avi-tagged Human Cell His\Avi
Mouse CD276-2881M Active Recombinant Mouse CD276, MIgG2a Fc-tagged CHO Fc
Mouse CD276-2881MAF488 Active Recombinant Mouse CD276 Protein, MIgG2a mFc-tagged, Alexa Fluor 488 conjugated CHO mFc
Mouse CD276-2881MAF647 Active Recombinant Mouse CD276 Protein, MIgG2a mFc-tagged, Alexa Fluor 647 conjugated CHO mFc
Mouse CD276-2881MAF555 Active Recombinant Mouse CD276 Protein, MIgG2a mFc-tagged, Alexa Fluor 555 conjugated CHO mFc
Mouse CD276-2881MF Active Recombinant Mouse CD276 Protein, MIgG2a mFc-tagged, FITC conjugated CHO mFc
Mouse CD276-941M Recombinant Mouse CD276 protein, His-tagged HEK293 His Met1-Phe244
Mouse Cd276-479M Recombinant Mouse Cd276 protein, His-tagged HEK293 His Val29-Phe244
Mouse Cd276-7675M Recombinant Mouse Cd276 protein, His-tagged E.coli His Thr47~Gly213
Mouse CD276-001MCL Recombinant Mouse CD276 cell lysate Human Cell N/A
Mouse CD276-1287MP Recombinant Mouse CD276 protein, Fc-tagged, R-PE labeled HEK293 Fc
Mouse CD276-601M Recombinant Mouse CD276 Protein HEK293 316
Mouse CD276-1287MAF488 Recombinant Mouse Cd276 Protein, hFc-tagged, Alexa Fluor 488 conjugated HEK293 hFc Val29-Phe244
Mouse CD276-2881MP Recombinant Mouse CD276 protein, MIgG2a Fc-tagged, R-PE labeled CHO MIgG2a Fc
Mouse CD276-2881MA Recombinant Mouse CD276 protein, MIgG2a Fc-tagged, APC labeled CHO MIgG2a Fc
Mouse CD276-602M Recombinant Mouse CD276 Protein, Fc-tagged HEK293 Fc 316
Mouse Cd276-479MAF647 Recombinant Mouse Cd276 Protein, His-tagged, Alexa Fluor 647 conjugated HEK293 His Val29-Phe244
Mouse Cd276-479MAF555 Recombinant Mouse Cd276 Protein, His-tagged, Alexa Fluor 555 conjugated HEK293 His Val29-Phe244
Mouse CD276-1287MF Recombinant Mouse Cd276 Protein, hFc-tagged, FITC conjugated HEK293 hFc Val29-Phe244
Mouse CD276-2905M Active Recombinant Mouse CD276 protein, Fc-tagged HEK293 Fc Val 29-Phe 244
Mouse CD276-1287MAF555 Recombinant Mouse Cd276 Protein, hFc-tagged, Alexa Fluor 555 conjugated HEK293 hFc Val29-Phe244
Mouse CD276-1287MAF647 Recombinant Mouse Cd276 Protein, hFc-tagged, Alexa Fluor 647 conjugated HEK293 hFc Val29-Phe244
Mouse Cd276-479MAF488 Recombinant Mouse Cd276 Protein, His-tagged, Alexa Fluor 488 conjugated HEK293 His Val29-Phe244
Mouse Cd276-5407M Recombinant Mouse Cd276 Protein (Val29-Phe244) Mammalian cells Tag Free Val29-Phe244
Mouse Cd276-1140M Recombinant Mouse Cd276 protein(Met1-Phe244), His-tagged, Biotinylated HEK293 C-His Met1-Phe244
Mouse CD276-1287M Recombinant Mouse CD276 protein, Fc-tagged HEK293 human/IgG1/Fc Val29-Phe244
Mouse CD276-1287MA Recombinant Mouse CD276 protein, Fc-tagged, APC labeled HEK293 Fc
Mouse Cd276-479MF Recombinant Mouse Cd276 Protein, His-tagged, FITC conjugated HEK293 His Val29-Phe244
Mouse Cd276-219M Recombinant Mouse Cd276 Protein, MYC/DDK-tagged HEK293T MYC/DDK
Rat CD276-1243R Recombinant Rat CD276 Protein Mammalian Cell His
Rat Cd276-40R Recombinant Rat Cd276 protein, hFc-tagged HEK293 hFc Met1-Phe244
Rat Cd276-41R Recombinant Rat Cd276, His tagged Human Cell His
Rat CD276-826RCL Recombinant Rat CD276 cell lysate Human Cell N/A
Rat Cd276-40RF Recombinant Rat Cd276 Protein, Fc-tagged, FITC conjugated HEK293 Fc 457
Rat CD276-901R Recombinant Rat CD276 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat Cd276-40RAF488 Recombinant Rat Cd276 Protein, Fc-tagged, Alexa Fluor 488 conjugated HEK293 Fc 457
Rat Cd276-40RP Recombinant Rat Cd276 protein, Fc-tagged, R-PE labeled HEK293 Fc
Rat Cd276-40RAF555 Recombinant Rat Cd276 Protein, Fc-tagged, Alexa Fluor 555 conjugated HEK293 Fc 457
Rat Cd276-41RAF555 Recombinant Rat Cd276 Protein, His-tagged, Alexa Fluor 555 conjugated HEK293 His 227
Rat Cd276-41RAF488 Recombinant Rat Cd276 Protein, His-tagged, Alexa Fluor 488 conjugated HEK293 His 227
Rat Cd276-40RAF647 Recombinant Rat Cd276 Protein, Fc-tagged, Alexa Fluor 647 conjugated HEK293 Fc 457
Rat Cd276-41RAF647 Recombinant Rat Cd276 Protein, His-tagged, Alexa Fluor 647 conjugated HEK293 His 227
Rat CD276-901R-B Recombinant Rat CD276 Protein Pre-coupled Magnetic Beads HEK293
Rat Cd276-40RA Recombinant Rat Cd276 protein, Fc-tagged, APC labeled HEK293 Fc
Rat Cd276-41RF Recombinant Rat Cd276 Protein, His-tagged, FITC conjugated HEK293 His 227
Monkey CD276-1235CAF555 Recombinant Monkey CD276 Protein, Fc-tagged, Alexa Fluor 555 conjugated HEK293 Fc 675
Monkey CD276-1235CAF488 Recombinant Monkey CD276 Protein, Fc-tagged, Alexa Fluor 488 conjugated HEK293 Fc 675
Monkey CD276-1235CF Recombinant Monkey CD276 Protein, Fc-tagged, FITC conjugated HEK293 Fc 675
Monkey CD276-1235CAF647 Recombinant Monkey CD276 Protein, Fc-tagged, Alexa Fluor 647 conjugated HEK293 Fc 675
Rhesus monkey CD276-1516R Recombinant Rhesus Monkey CD276 Protein, hIgG4-tagged HEK293 hIgG4
Cynomolgus CD276-1355C Recombinant Cynomolgus CD276 protein, His-tagged HEK293 His Leu29-Glu465
Cynomolgus Monkey CD276-1235C Recombinant Cynomolgus CD276 Protein, Fc-tagged HEK293 Fc
Zebrafish CD276-5113Z Recombinant Zebrafish CD276 Mammalian Cell His
Chicken CD276-5542C Recombinant Chicken CD276 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource

What is CD276 protein?

CD276 (CD276 molecule) gene is a protein coding gene which situated on the long arm of chromosome 15 at locus 15q24. The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. The CD276 protein is consisted of 534 amino acids and its molecular mass is approximately 57.2 kDa.

What is the function of CD276 protein?

CD276 acts as an immune checkpoint molecule and is selectively expressed in both tumor cells and immune cells within the tumor microenvironment. CD276 is implicated in the regulation of glucose uptake and metabolism in tumor cells, supporting the Warburg effect, which is a metabolic characteristic of cancer cells. CD276 promotes angiogenesis by stimulating the secretion of vascular endothelial growth factor (VEGF), which is crucial for the growth and survival of tumors. While CD276 mRNA is broadly expressed in various tissues, protein expression is not ubiquitous, suggesting post-transcriptional regulation mechanisms. CD276 is a highly glycosylated protein, and its post-translational modifications, such as glycosylation, are important for its function and stability on the cell surface.

The central role of B7-H3 on factors regulating tumor cell plasticity and tumorigenicity.jpg

Fig1. The central role of B7-H3 on factors regulating tumor cell plasticity and tumorigenicity. (Elizabeth Varghese, 2024)

CD276 Related Signaling Pathway

CD276 has been shown to interact with the phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) pathway, which is known to regulate cell survival, growth, and metabolism. Activation of this pathway by CD276 can lead to the suppression of immune cell activity and promotion of tumor cell survival. CD276 is mentioned as an immune checkpoint molecule in the epithelial-mesenchymal transition (EMT) pathway, which is involved in the development and metastasis of carcinomas. CD276 has been linked to the regulation of metabolic pathways in tumor cells, which can support tumor growth and survival through altered energy metabolism. CD276 deletion in TAMs leads to downregulation of chemokine signaling pathways, which are important for immune cell recruitment and infiltration into the tumor.

CD276 Related Diseases

CD276 is highly expressed in bladder cancer and is linked to poorer overall survival and progression-free survival in patients. Its expression is associated with diminished tumor-infiltrating lymphocytes and hastened cancer progression. Targeting CD276 has shown potential as a therapeutic strategy for bladder tumors. CD276 expression on tumor-associated endothelial cells in ovarian carcinoma is associated with poor patient outcomes. D276 is mentioned in the context of asthma, where it is associated with allergic diseases and the immune response. CD276 is associated with neuroblastoma, which is a cancer that arises from nerve cells, and is linked to the development and differentiation of neural crest cells. CD276 may play a role in this immune condition that occurs after a bone marrow transplant.

Bioapplications of CD276

CD276 blockade in combination with other immune checkpoint inhibitors, such as PD-1/PD-L1 and CTLA-4, is being explored to enhance the efficacy of cancer immunotherapy, particularly in tumors that do not respond well to single-agent treatments. Due to its elevated expression in various solid tumors and its role in promoting an immunosuppressive tumor microenvironment, CD276 is being explored as a therapeutic target. Monoclonal antibodies and bispecific antibodies targeting CD276 are being developed to disrupt its immunosuppressive functions.

High Purity

SDS PAGE (CD276-053H).jpg

Fig1. SDS-PAGE (CD276-053H)

.

SDS PAGE (CD276-303H).jpg

Fig2. SDS-PAGE (CD276-303H)

Case Study 1: Margie N Sutton, 2024

B7-H3 (CD276) has two isoforms (2Ig and 4Ig), no confirmed cognate receptor, and physiological functions that remain elusive. While differentially expressed on many solid tumors correlating with poor survival, mechanisms of how B7-H3 signals in cis (tumor cell) versus in trans (immune cell co-regulator) to elicit pro-tumorigenic phenotypes remain poorly defined. Herein, the researchers characterized a tumorigenic and signaling role for tumor cell-expressed 4Ig-B7-H3, the dominant human isoform, in gynecological cancers that could be abrogated upon CRISPR/Cas9 knockout of B7-H3; tumorigenesis was rescued upon re-expression of 4Ig-B7-H3. Size exclusion chromatography revealed dimerization states for the extracellular domains of both human 4Ig- and murine 2Ig-B7-H3. mEGFP lifetimes of expressed 4Ig-B7-H3-mEGFP fusions determined by FRET-FLIM assays confirmed close-proximity interactions of 4Ig-B7-H3 and identified two distinct homo-FRET lifetime populations, consistent with monomeric and homo-dimer interactions. In live cells, bioluminescence imaging of 4Ig-B7-H3-mediated split luciferase complementation showed dimerization of 4Ig-B7-H3.

HeLa CD276 cells engineered were used to perform a clonogenic assay.jpg

Fig1. HeLa CD276-/- cells engineered to re-express 4Ig-B7-H3 (LV B7-H3) or a negative vector control (LV Neg) were used to perform a clonogenic assay.

Chromatograms of human 4Ig-B7-H3 and murine 2Ig-B7-H3 with their calculated molecular weights.jpg

Fig2. Chromatograms of human 4Ig-B7-H3 and murine 2Ig-B7-H3 with their calculated molecular weights.

Case Study 2: Haiyan Jin, 2023

Ferroptosis, a newly discovered form of regulated cell death, has been reported to be associated with multiple cancers, including colorectal cancer (CRC). However, the underlying molecular mechanism is still unclear. In this study, the researchers identified B7H3 as a potential regulator of ferroptosis resistance in CRC. B7H3 knockdown decreased but B7H3 overexpression increased the ferroptosis resistance of CRC cells, as evidenced by the expression of ferroptosis-associated genes (PTGS2, FTL, FTH, and GPX4) and the levels of important indicators of ferroptosis (malondialdehyde, iron load). Moreover, B7H3 promoted ferroptosis resistance by regulating sterol regulatory element binding protein 2 (SREBP2)-mediated cholesterol metabolism. Both exogenous cholesterol supplementation and treatment with the SREBP2 inhibitor betulin reversed the effect of B7H3 on ferroptosis in CRC cells. Furthermore, the researchers verified that B7H3 downregulated SREBP2 expression by activating the AKT pathway. Additionally, multiplex immunohistochemistry was carried out to show the expression of B7H3, prostaglandin-endoperoxide synthase 2, and SREBP2 in CRC tumor tissues, which was associated with the prognosis of patients with CRC.

B7H3 protein levels were analyzed by western blotting.jpg

Fig3. B7H3 protein levels were analyzed by western blotting.

Volumes of B7H3-knockdown HCT116 tumors in nude mice fed a high cholesterol diet.jpg

Fig4. Volumes of B7H3-knockdown HCT116 tumors in nude mice fed a high cholesterol diet.

B7-H3-related immunosuppressive pathways in colorectal cancer.jpg

Fig1. B7-H3-related immunosuppressive pathways in colorectal cancer. (Huan Zhang, 2024)

The mechanisms whereby B7-H3 promotes the invasion and metastasis of colorectal cancer.jpg

Fig2. The mechanisms whereby B7-H3 promotes the invasion and metastasis of colorectal cancer. (Huan Zhang, 2024)

CD276 involved in several pathways and played different roles in them. We selected most pathways CD276 participated on our site, such as Cell adhesion molecules (CAMs), which may be useful for your reference. Also, other proteins which involved in the same pathway with CD276 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Cell adhesion molecules (CAMs)HLA-DPA1;NRCAM;CLDN7B;NRXN1;CLDN10;NLGN2;CLDN19;NRXN2;ITGB7

CD276 has several biochemical functions, for example, protein binding, receptor binding. Some of the functions are cooperated with other proteins, some of the functions could acted by CD276 itself. We selected most functions CD276 had, and list some proteins which have the same functions with CD276. You can find most of the proteins on our site.

Function Related Protein
protein bindingSCO2;SUGP1;CEP72;SLC6A20;BRK1;DNAI1;TDP1;PIP;ZNF496
receptor bindingEPB49;CADM1B;SERPINE1;SRPX2;CRH;NRXN1;PLA2G1B;APP;Icosl

CD276 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with CD276 here. Most of them are supplied by our site. Hope this information will be useful for your research of CD276.

ganglioside_gm1; cona_canen

Research Area

Related articles

Wang, L; Kang, FB; et al. B7-H3-mediated tumor immunology: Friend or foe?. INTERNATIONAL JOURNAL OF CANCER 134:2764-2771(2014).
Lutz, AM; Bachawal, SV; et al. Ultrasound Molecular Imaging in a Human CD276 Expression-Modulated Murine Ovarian Cancer Model. CLINICAL CANCER RESEARCH 20:1313-1322(2014).
  • Q&As
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Q&As (5)

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In which other diseases, besides cancer, is CD276 implicated? 11/29/2022

CD276 has been associated with autoimmune diseases and certain inflammatory conditions. Exploring its role in these diseases may open avenues for therapeutic interventions.

Are there diagnostic applications for CD276 in cancer detection? 10/15/2021

Research suggests that CD276 expression levels may serve as a diagnostic biomarker for certain cancers. Detection methods, such as immunohistochemistry, are being explored to assess CD276 levels in tumor tissues.

How does CD276 influence the tumor microenvironment? 05/07/2020

CD276 can modulate the tumor microenvironment by suppressing immune responses, promoting angiogenesis, and supporting tumor cell survival. Understanding these interactions is crucial for developing targeted therapies.

Are there any ongoing clinical trials focusing on CD276 in cancer patients? 06/20/2016

Yes, several clinical trials are underway to evaluate the safety and efficacy of CD276-targeted therapies in different cancer types. These trials aim to assess the impact on patient outcomes and potential side effects.

How does CD276-targeted therapy differ from traditional cancer treatments? 02/14/2016

CD276-targeted therapy focuses on modulating the immune response against cancer cells, whereas traditional treatments like chemotherapy and radiation target rapidly dividing cells, including both healthy and cancerous cells.

Customer Reviews (3)

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Reviews
03/15/2022

    It has consistently demonstrated high sensitivity and accuracy in detecting and quantifying target molecules, allowing for precise and reliable measurement of analytes in my experimental samples.

    08/06/2021

      Its versatility and reliability make it an excellent choice for research applications, and I highly recommend it to fellow scientists.

      06/03/2021

        The sensitivity and specificity of the CD276 Protein have consistently delivered robust and consistent results, enhancing the efficiency and accuracy of my experimental workflows.

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