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Active Recombinant Human CD40 Ligand

Cat.No. : CD40LG-4737H
Product Overview : CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. Recombinant Human CD40L produced in E.Coli is a non-glycosylated, Polypeptide chain containing 149 amino acids and having a molecular mass of 16308 Dalton.
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  • Gene Information
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Cat. No. : CD40LG-4737H
Description : CD154, also called CD40 ligand or CD40L, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. In general, CD40L plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor sti-mulation by MHC molecules on the APC.
Species : Human
Source : E.coli
Form : CD40L is lyophilized from 10mM Sodium Phosphate pH=7.5.
Purity : Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity : H-CD40L is fully biologically active when compared to standard. The ED50 as determined using T-47D cells is <0.04 ug/ml.
Physical Appearance : Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility : Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml which can be further diluted into other aqueous solutions.
Purity Greater than 98% as determined by : Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by : UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level : Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Amino acid sequence : MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
Storage : Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Gene Name : CD40LG CD40 ligand [ Homo sapiens ]
Official Symbol : CD40LG
Synonyms : CD40LG; CD40 ligand; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L; CD40 ligand; T-cell antigen Gp39; T-B cell-activating molecule; tumor necrosis factor (ligand) superfamily member 5; TNF-related activation protein; CD40 antigen ligand; CD40-L
Gene ID : 959
mRNA Refseq : NM_000074
Protein Refseq : NP_000065
MIM : 300386
UniProt ID : P29965
Chromosome Location : Xq26
Pathway : Allograft rejection; Asthma; Autoimmune thyroid disease; Cell adhesion molecules (CAMs); Cytokine-cytokine receptor interaction; Intestinal immune network for IgA production; Systemic lupus erythematosus; T cell receptor signaling pathway; Viral myocarditis; Signaling in Immune system
Function : CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Are there any ongoing clinical trials targeting CD40LG for autoimmune diseases? 11/01/2022

Yes, there are clinical trials investigating the modulation of CD40LG in autoimmune diseases, exploring its potential role in regulating immune responses that contribute to autoimmunity.

Can CD40LG be a potential target for the treatment of infectious diseases? 02/27/2021

Yes, research suggests that CD40LG modulation might be a viable strategy in the development of treatments for infectious diseases by enhancing immune responses.

How does the interaction between CD40LG and its receptor CD40 influence antibody production? 11/20/2020

The interaction between CD40LG and CD40 is crucial for the activation of B cells, leading to the production of antibodies and the development of an effective immune response.

How is CD40LG involved in the regulation of inflammatory responses? 08/26/2020

CD40LG is involved in modulating inflammatory responses by influencing the activation and function of immune cells, contributing to the regulation of the immune system.

In what ways is CD40LG being explored for its potential in vaccine development? 04/20/2020

CD40LG is being investigated as an adjuvant in vaccine development, aiming to enhance the immune response and improve the efficacy of vaccines against various diseases.

Customer Reviews (3)

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Reviews
10/17/2022

    The reliable performance of the CD40LG protein in ELISA and its compatibility with protein electron microscopy structure analysis make it an excellent choice for a wide range of research studies.

    01/01/2022

      Its reliable and accurate performance ensures dependable results, contributing to the advancement of scientific knowledge in various fields of study.

      11/16/2020

        Their knowledgeable and responsive team assists in troubleshooting and finding solutions to any experimental challenges that may arise.

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